中性粒細胞彈性蛋白酶(NE)重組蛋白
Recombinant Neutrophil Elastase (NE)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
- 編號URPA181Hu03
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量37.0kDa
- 實際分子量40kDa(差異分析請參閱說明書)
- 片段與標簽Ile30~Gln247 with Two N-terminal Tags, His-tag and SUMO-tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點8.7
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 362 ¥ 906 ¥ 1812 ¥ 5436 ¥ 13590
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA181Hu02 | 中性粒細胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA181Hu03 | 中性粒細胞彈性蛋白酶(NE)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA181Hu01 | 中性粒細胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA181Hu03 | 中性粒細胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA181Hu04 | 中性粒細胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA181Hu01 | 中性粒細胞彈性蛋白酶(NE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA181Hu01 | 中性粒細胞彈性蛋白酶(NE)多克隆抗體 | WB; IHC |
| UMAA181Hu22 | 中性粒細胞彈性蛋白酶(NE)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA181Hu | 中性粒細胞彈性蛋白酶(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA181Hu | 中性粒細胞彈性蛋白酶(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA181Hu | 中性粒細胞彈性蛋白酶(NE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Journal of Photochemistry and Photobiology B: Biology | A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262] |
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| Clin Exp Pharmacol Physiol. | Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688] |
| Journal of Cardiology | Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction [10.1016:j.jjcc.2018.06.003] |
| Neuroscience | Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats [Pubmed:29684507] |
| Molecular and Cellular Biochemistry | Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes [Pubmed:28948423] |
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| Cell Death & Disease | Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation [Pubmed: 31515489] |
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| Journal of Clinical Immunology | MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes [Pubmed: 31749032] |
| Pathology & Oncology Research | Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer [Pubmed: 31656990] |
| Bioscience Reports | Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats [Pubmed: 31763669] |
| Oxidative Medicine and Cellular Longevity | Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation [Pubmed: 31871555] |
| Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis [Pubmed: 31927460] |
| Journal of neural transmission (Vienna, Austria : 1996) | Neurotransmitter system aberrations in patients with drug addiction [Pubmed: 32804296] |
| experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis [Pubmed: 32376471] |
| CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats [Pubmed: 32349626] |
| Br J Pharmacol | Macrophage Extracellular Traps Aggravate Iron Overload?\Related Liver Ischemia/Reperfusion Injury [33959955] |
| Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling??- [33505502] |
| Nat Sci Sleep | Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with?… [34934375] |
| Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome [Pubmed:35088760] |
| A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation [] |
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