中性粒細(xì)胞彈性蛋白酶(NE)多克隆抗體
Polyclonal Antibody to Neutrophil Elastase (NE)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
- 編號(hào)UPAA181Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPA181Hu01-中性粒細(xì)胞彈性蛋白酶(NE)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種-
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ELA2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Rectum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ELA2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Vector Red staining on IHC-P;
Samples: Human Spleen Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ELA2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human NE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Human Leukocyte lysate; Lane2: Human peripheral blood mononuclear cell; Primary Ab: 0.2μg/ml Rabbit Anti-Human NE Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)NE的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別NE。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA181Hu02 | 中性粒細(xì)胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA181Hu03 | 中性粒細(xì)胞彈性蛋白酶(NE)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA181Hu01 | 中性粒細(xì)胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA181Hu03 | 中性粒細(xì)胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA181Hu04 | 中性粒細(xì)胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA181Hu01 | 中性粒細(xì)胞彈性蛋白酶(NE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA181Hu01 | 中性粒細(xì)胞彈性蛋白酶(NE)多克隆抗體 | WB; IHC |
| UMAA181Hu22 | 中性粒細(xì)胞彈性蛋白酶(NE)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA181Hu | 中性粒細(xì)胞彈性蛋白酶(NE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA181Hu | 中性粒細(xì)胞彈性蛋白酶(NE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA181Hu | 中性粒細(xì)胞彈性蛋白酶(NE)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Mediators of Inflammation | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation [Hindawi: 612848] |
| Journal of Photochemistry and Photobiology B: Biology | A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262] |
| International Journal of Molecular Medicine | The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats [pubmed:27430552] |
| European?Journal?of?Trauma?and?Emergency | Xuebijing?injection?treatment?inhibits?vasopermeability?and?reduces?fluid?requirements?in acanine?burn?model. [pubmed:28070608] |
| Clin Exp Pharmacol Physiol. | Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688] |
| Journal of Cardiology | Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction [10.1016:j.jjcc.2018.06.003] |
| Neuroscience | Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats [Pubmed:29684507] |
| Molecular and Cellular Biochemistry | Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes [Pubmed:28948423] |
| Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic?… [Pubmed:29379286] |
| Behavioural?Brain?Research | Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats [Pubmed: 30423388] |
| Journal of?Pineal?Research | Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267] |
| Fundamental & clinical pharmacology | Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo [Pubmed: 31313350] |
| Cell Death & Disease | Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation [Pubmed: 31515489] |
| Journal of nutritional science and vitaminology | Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively?… [Pubmed: 31474682] |
| Journal of Clinical Immunology | MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes [Pubmed: 31749032] |
| Pathology & Oncology Research | Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer [Pubmed: 31656990] |
| Bioscience Reports | Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats [Pubmed: 31763669] |
| Oxidative Medicine and Cellular Longevity | Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation [Pubmed: 31871555] |
| Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis [Pubmed: 31927460] |
| Journal of neural transmission (Vienna, Austria : 1996) | Neurotransmitter system aberrations in patients with drug addiction [Pubmed: 32804296] |
| experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis [Pubmed: 32376471] |
| CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats [Pubmed: 32349626] |
| Br J Pharmacol | Macrophage Extracellular Traps Aggravate Iron Overload?\Related Liver Ischemia/Reperfusion Injury [33959955] |
| Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling??- [33505502] |
| Nat Sci Sleep | Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with?… [34934375] |
| Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome [Pubmed:35088760] |
| A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation [] |
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