載脂蛋白A1(APOA1)重組蛋白
Recombinant Apolipoprotein A1 (APOA1)
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
- 編號(hào)URPA519Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU per 1μg (determined by the LAL method).
- 亞細(xì)胞定位分泌
- 預(yù)測(cè)分子量18.5kDa
- 實(shí)際分子量17kDa(差異分析請(qǐng)參閱說明書)
- 片段與標(biāo)簽Gln122~Gln267 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.4
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 360 ¥ 900 ¥ 1800 ¥ 5400 ¥ 13500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA519Hu01 | 載脂蛋白A1(APOA1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA519Hu61 | 載脂蛋白A1(APOA1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA519Hu01 | 載脂蛋白A1(APOA1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA519Hu61 | 載脂蛋白A1(APOA1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA519Hu06 | 載脂蛋白A1(APOA1)多克隆抗體 | WB |
| UPAA519Hu01 | 載脂蛋白A1(APOA1)多克隆抗體 | WB |
| ULAA519Hu71 | 載脂蛋白A1(APOA1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA519Hu21 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC |
| URAA519Hu91 | 載脂蛋白A1(APOA1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA519Hu24 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC |
| UMAA519Hu26 | 載脂蛋白A1(APOA1)單克隆抗體 | WB |
| UMAA519Hu23 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA519Hu25 | 載脂蛋白A1(APOA1)單克隆抗體 | WB |
| UMAA519Hu29 | 載脂蛋白A1(APOA1)單克隆抗體 | ICC/IF |
| URAA519Hu21 | 載脂蛋白A1(APOA1)重組抗體 | WB; IHC |
| URAA519Hu27 | 載脂蛋白A1(APOA1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA519Hu22 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC |
| UFAA519Hu02 | 抗載脂蛋白A1(APOA1)單克隆抗體 | FCM |
| ULAA519Hu76 | 載脂蛋白A1(APOA1)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA519Hu | 載脂蛋白A1(APOA1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA519Hu | 抗載脂蛋白A1抗體(Anti-APOA1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCA519Hu | 載脂蛋白A1(APOA1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA519Hu | 載脂蛋白A1(APOA1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA519Hu01 | 載脂蛋白A1(APOA1)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA519Hu01 | 載脂蛋白A1(APOA1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| European Journal of Applied Physiology | Endurance training enhances ABCA1 expression in rat small intestine [PubMed: 19629515] |
| Official Journal of the INWR | Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. [Wrestling: source] |
| Biochemical and Biophysical Research Communications | Treadmill exercise enhances ABCA1 expression in rat liver [ScienceDirect: S0006291X0701488X] |
| World Journal of Sport Sciences | The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1 [Idosi: source] |
| Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ] |
| Biomarkers | The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry study [PubMed: 22458349] |
| Journal of the American Heart Association | MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice [PubMed: PMC3540673] |
| PLoS ONE | Apolipoprotein AI Is a Potential Mediator of Remote Ischemic Preconditioning [Plosone: Source] |
| Molecular Genetics and Metabolism | DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levels [Pubmed: 23920041] |
| Endocrinology | Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice [Pubmed: 24684302] |
| Molecular Genetics and Metabolism Reports | Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia [Sciencedirect:S2214426915000026] |
| J Psychopharmacol | Anti-atherogenic properties of high-density lipoproteins in psychiatric patients before and after two months of atypical anti-psychotic therapy [PubMed: 26253619] |
| Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
| Pharmacol Res Perspect | Efficacy of tomato concentrates in mouse models of dyslipidemia and cancer [PubMed: 26171234] |
| Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173] |
| Oncogene | Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis [PubMed: 26279300] |
| Oncogene | Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesis [Pubmed:26279300] |
| Allergy | Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis. [pubmed:28378321] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| 11 | High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signaling [pubmed:27866837] |
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| ACS?Biomaterials Science?&?Engineering | Enhanced Antiatherosclerotic Efficacy of Statin-Loaded Reconstituted High-Density Lipoprotein via Ganglioside GM1 Modification [abs:10.1021] |
| Food?Research International | Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541] |
| Journal of The Science of Food and Agriculture | Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773] |
| Toxicon | A membrane disrupting toxin from wasp venom underlies the molecular mechanism of tissue damage [Pubmed:29654869] |
| European?Journal of Pharmacology | The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981] |
| European Journal of Preventive Cardiology | High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats [Pubmed: 31718266] |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
| VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| Cardiovasc Toxicol | Interplay of Obesity, Ethanol, and Contaminant Mixture on Clinical Profiles of Cardiovascular and Metabolic Diseases: Evidence from an Animal Study [Pubmed:35429258] |
| Peptides | Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor [Pubmed:35660638] |
| Journal of Functional Foods | Castanea mollissima shell polyphenols regulate JAK2 and PPARγ expression to suppress inflammation and lipid accumulation by inhibiting M1 macrophages?… [] |
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