載脂蛋白A1(APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Apolipoprotein A1 (APOA1)
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
- 編號USEA519Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍6.25-400ng/mL
- 靈敏度最小可檢測劑量小于等于2.59ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1151 ¥ 1644 ¥ 7398 ¥ 13974 ¥ 115080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測載脂蛋白A1(APOA1)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的載脂蛋白A1(APOA1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-95 | 80 |
| EDTA plasma(n=5) | 92-101 | 96 |
| heparin plasma(n=5) | 93-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的載脂蛋白A1(APOA1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中載脂蛋白A1(APOA1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-105% | 91-99% | 93-101% | 95-103% |
| EDTA plasma(n=5) | 88-105% | 94-105% | 96-103% | 85-101% |
| heparin plasma(n=5) | 79-95% | 88-101% | 96-105% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將載脂蛋白A1(APOA1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的載脂蛋白A1(APOA1)與連接于固相載體上的抗體結合，然后加入生物素化的載脂蛋白A1(APOA1)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的載脂蛋白A1(APOA1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA519Hu01 | 載脂蛋白A1(APOA1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA519Hu61 | 載脂蛋白A1(APOA1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA519Hu01 | 載脂蛋白A1(APOA1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA519Hu61 | 載脂蛋白A1(APOA1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA519Hu06 | 載脂蛋白A1(APOA1)多克隆抗體 | WB |
| UPAA519Hu01 | 載脂蛋白A1(APOA1)多克隆抗體 | WB |
| ULAA519Hu71 | 載脂蛋白A1(APOA1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA519Hu21 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC |
| URAA519Hu91 | 載脂蛋白A1(APOA1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA519Hu24 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC |
| UMAA519Hu26 | 載脂蛋白A1(APOA1)單克隆抗體 | WB |
| UMAA519Hu23 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA519Hu25 | 載脂蛋白A1(APOA1)單克隆抗體 | WB |
| UMAA519Hu29 | 載脂蛋白A1(APOA1)單克隆抗體 | ICC/IF |
| URAA519Hu21 | 載脂蛋白A1(APOA1)重組抗體 | WB; IHC |
| URAA519Hu27 | 載脂蛋白A1(APOA1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA519Hu22 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC |
| UFAA519Hu02 | 抗載脂蛋白A1(APOA1)單克隆抗體 | FCM |
| ULAA519Hu76 | 載脂蛋白A1(APOA1)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA519Hu | 載脂蛋白A1(APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA519Hu | 抗載脂蛋白A1抗體(Anti-APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCA519Hu | 載脂蛋白A1(APOA1)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA519Hu | 載脂蛋白A1(APOA1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA519Hu01 | 載脂蛋白A1(APOA1)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA519Hu01 | 載脂蛋白A1(APOA1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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| Official Journal of the INWR | Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. [Wrestling: source] |
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| World Journal of Sport Sciences | The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1 [Idosi: source] |
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