過氧化氫酶(CAT)活性蛋白
Active Catalase (CAT)
- 編號UAPC418Hu05
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 486 ¥ 1215 ¥ 2430 ¥ 7290 ¥ 18225
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. As we know, H2O2 and (NH4)2MoO4 can produce a stable yellow product which has a absorbance at 405 nm. Thus, the recombinant human CAT activity was measured by its ability to hydrolyze H2O2 to H2O , the remaining H2O2 in the reaction was measured by (NH4)2MoO4. The reaction was performed in 60 mM phosphate buffer pH 7.4 (Assay Buffer), initiated by addition 100 μL of various concentrations of CAT (diluted by Assay Buffer) to 1 mL of 65 mM Substrate H2O2 (diluted by Assay Buffer). Incubated at 37℃ for 1min, then add 1 ml 200 mM (NH4)2MoO4. After 10 minutes at room temperature, read at a wavelength of 405 nm.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC418Hu03 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC418Hu02 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC418Hu05 | 過氧化氫酶(CAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC418Hu05 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC418Hu04 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC418Hu02 | 過氧化氫酶(CAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC418Hu01 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC418Hu03 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; FCM |
| UPAC418Hu01 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| UPAC418Hu05 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; FCM |
| UPAC418Hu04 | 過氧化氫酶(CAT)多克隆抗體 | WB; ICC/IF; FCM |
| UPAC418Hu02 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAC418Hu71 | 過氧化氫酶(CAT)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAC418Hu81 | 過氧化氫酶(CAT)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAC418Hu21 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| UMAC418Hu27 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu29 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu22 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAC418Hu24 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAC418Hu23 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu25 | 過氧化氫酶(CAT)單克隆抗體 | ICC/IF |
| UMAC418Hu26 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| UMAC418Hu28 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| USEC418Hu | 過氧化氫酶(CAT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC418Hu | 過氧化氫酶(CAT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC418Hu01 | 過氧化氫酶(CAT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
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| Molecular Medicine Reports | MicroRNA?21 contributes to the puerarin?induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury [Pubmed: 31115556] |
| Advances in Medical Sciences | Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169] |
| European Journal of Biomedical and Pharmaceutical Sciences | European Journal of Biomedical AND Pharmaceutical sciences [] |
| Anticancer Research | The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer [Pubmed: 31892574] |
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