過氧化氫酶(CAT)多克隆抗體
Polyclonal Antibody to Catalase (CAT)
- 編號UPAC418Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPC418Hu01-過氧化氫酶(CAT)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Felis catus; Feline (Cat，貓)
- 應用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 252 ¥ 588 ¥ 840 ¥ 2100 ¥ 8400
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CAT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Kidney lysate; Lane3: Mouse Liver lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate Primary Ab: 0.04μg/ml Rabbit Anti-Human CAT Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: CAT knockout HepG2 cell lysate;
Predicted MW: 60kd
Observed MW: 60kd
Primary Ab: 2μg/ml Rabbit Anti-Human CAT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human THP1 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human CAT Polyclonal Antibody (Catalog PAC418Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對CAT的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別CAT。
用法
Western blotting:0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPC418Hu03 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC418Hu02 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC418Hu05 | 過氧化氫酶(CAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC418Hu05 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC418Hu04 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC418Hu02 | 過氧化氫酶(CAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC418Hu01 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC418Hu03 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; FCM |
| UPAC418Hu01 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| UPAC418Hu05 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; FCM |
| UPAC418Hu04 | 過氧化氫酶(CAT)多克隆抗體 | WB; ICC/IF; FCM |
| UPAC418Hu02 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAC418Hu71 | 過氧化氫酶(CAT)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| ULAC418Hu81 | 過氧化氫酶(CAT)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAC418Hu21 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| UMAC418Hu27 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu29 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu22 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAC418Hu24 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAC418Hu23 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu25 | 過氧化氫酶(CAT)單克隆抗體 | ICC/IF |
| UMAC418Hu26 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| UMAC418Hu28 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| USEC418Hu | 過氧化氫酶(CAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC418Hu | 過氧化氫酶(CAT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC418Hu01 | 過氧化氫酶(CAT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
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| Journal of Food Science and Technology | Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats [Springer: Source] |
| 第 三 軍 醫(yī) 大 學 學 報 | 過氧化氫酶基因 ３８９Ｃ＞Ｔ多態(tài)位點在重慶漢族人群中的分布及其與噪音性耳聾的 關聯(lián)研究 [Tmmu:Source] |
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| Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes [PubMed: 26143097] |
| J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886] |
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| Pharmacological Reports | Evaluation of ADMA, carbonyl groups, CAT and NKA in depressed patients with and without posttraumatic stress disorder. [pubmed:28554099] |
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| Journal of?Cosmetic?Dermatology | A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signaling [Pubmed:30047218] |
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| Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002] |
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| European Journal of Biomedical and Pharmaceutical Sciences | European Journal of Biomedical AND Pharmaceutical sciences [] |
| Anticancer Research | The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer [Pubmed: 31892574] |
| PHARMACOLOGICAL RESEARCH | A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling [Pubmed: 32135248] |
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| AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS [] | |
| The Journal of Physiology | In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells [] |
| Journal of Cell Communication and Signaling | Lutein reverses hyperglycemia-mediated blockage of Nrf2 translocation by modulating the activation of intracellular protein kinases in retinal pigment epithelial (ARPE?… [] |
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