血小板因子4(PF4)重組蛋白
Recombinant Platelet Factor 4 (PF4)
CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
- 編號(hào)URPA172Mu01
 - 物種Mus musculus (Mouse,小鼠) 相同的名稱(chēng),不同的物種。
 - 來(lái)源原核表達(dá)
 - 宿主E.coli
 - 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
 - 亞細(xì)胞定位分泌
 - 預(yù)測(cè)分子量12.0kDa
 - 實(shí)際分子量13kDa(差異分析請(qǐng)參閱說(shuō)明書(shū))
 - 片段與標(biāo)簽Val30~Ser105 with N-terminal His Tag
 - 緩沖液成份磷酸鹽緩沖液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
 - 性狀凍干粉
 - 純度> 90%
 - 等電點(diǎn)9.4
 - 
                    應(yīng)用
                    Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
 - 規(guī)格 10μg50μg 200μg 1mg 5mg
 - 價(jià)格 ¥ 540 ¥ 1350 ¥ 2700 ¥ 8100 ¥ 20250
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序列
                                
                            用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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| 編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) | 
| UAPA172Mu01 | 血小板因子4(PF4)活性蛋白 | Cell?culture;?Activity?Assays. | 
| URPA172Mu01 | 血小板因子4(PF4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| URPA172Mu02 | 血小板因子4(PF4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| UPAA172Mu01 | 血小板因子4(PF4)多克隆抗體 | WB; IHC; ICC; IP. | 
| UPAA172Mu02 | 血小板因子4(PF4)多克隆抗體 | WB; IHC | 
| ULAA172Mu71 | 血小板因子4(PF4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. | 
| ULAA172Mu81 | 血小板因子4(PF4)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. | 
| USEA172Mu | 血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| USCA172Mu | 血小板因子4(PF4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. | 
| ULMA172Mu | 血小板因子4(PF4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. | 
| UKSA172Mu01 | 血小板因子4(PF4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". | 
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) | 
| Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics | Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats [PubMed: 20601223] | 
| Basic Research in Cardiology | Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170] | 
| PLoS One | Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation [PubMed: PMC3608633] | 
| Diving and Hyperbaric Medicine | Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression [Pubmed:25311322] | 
| Journal of Functional Foods | A?Phellinus baumii–based supplement containing?Salvia miltiorrhiza?Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro?and?in vivo [science:S1756464616300676] | 
| Plos one | Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury [pubmed:27583400] | 
| international journal of molecular sciences | Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. [pubmed:28272338] | 
| Experimental?and?Therapeutic?Medicine | Feasibility of improving platelet?rich plasma therapy by using chitosan with high platelet activation ability [pubmed:28450960] | 
| Reproductive Toxicology | Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats [Pubmed: 30528429] | 
| INTERNATIONAL JOURNAL OF CARDIOLOGY | Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation [Pubmed: 30857843] | 
| Available at SSRN 3335365? | Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers [] | 
| journal of biotechnology | Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis [Pubmed: 30927490] | 
| Data in Brief | Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation [] | 
| journal of thoracic and cardiovascular surgery | Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers [Pubmed: 32812629] | 
| Marine Drugs | The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230] | 
| Thrombosis Research | Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449] | 
| Nat Commun | The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets [34099640] | 
| Vaccine | The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388] | 

                    
                    
                                