腦啡肽酶(CD10)單克隆抗體
Monoclonal Antibody to Neprilysin (CD10)
NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase
- 編號UMAB785Hu25
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C8
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB785Hu01-腦啡肽酶(CD10)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.54mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 335 ¥ 781 ¥ 1116 ¥ 2790 ¥ 11160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 30μg/ml Mouse Anti-Human NEP Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Pancreas Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human NEP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Porcine Kidney lysate; Lane2: Rat Kidney lysate Primary Ab: 0.2μg/ml Mouse Anti-Human IGF1 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對CD10的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CD10。
用法
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPB785Hu61 | 腦啡肽酶(CD10)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB785Hu02 | 腦啡肽酶(CD10)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB785Hu01 | 腦啡肽酶(CD10)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB785Hu61 | 腦啡肽酶(CD10)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB785Hu01 | 腦啡肽酶(CD10)多克隆抗體 | WB; IHC |
| UPAB785Hu02 | 腦啡肽酶(CD10)多克隆抗體 | WB; IHC |
| ULAB785Hu71 | 腦啡肽酶(CD10)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAB785Hu21 | 腦啡肽酶(CD10)單克隆抗體 | WB; ICC/IF |
| UMAB785Hu23 | 腦啡肽酶(CD10)單克隆抗體 | WB |
| UMAB785Hu24 | 腦啡肽酶(CD10)單克隆抗體 | WB |
| UMAB785Hu22 | 腦啡肽酶(CD10)單克隆抗體 | WB |
| UMAB785Hu25 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC |
| UMAB785Hu27 | 腦啡肽酶(CD10)單克隆抗體 | WB |
| UMAB785Hu29 | 腦啡肽酶(CD10)單克隆抗體 | WB |
| UMAB785Hu28 | 腦啡肽酶(CD10)單克隆抗體 | WB; ICC/IF |
| UMAB785Hu26 | 腦啡肽酶(CD10)單克隆抗體 | WB |
| UFAB785Hu02 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| UFAB785Hu04 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| UFAB785Hu06 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| UFAB785Hu08 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| UFAB785Hu00 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| UFAB785Hu82 | 抗腦啡肽酶(CD10)單克隆抗體(異硫氰酸熒光素標(biāo)記) | FCM |
| USEB785Hu | 腦啡肽酶(CD10)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB785Hu | 腦啡肽酶(CD10)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| 13 | Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme Inhibitors [Ahajournals:Source] |
| Biol Pharm Bull | Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty Rats [PubMed: 25832640] |
| Eur J Heart Fail.? | Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fraction [Pubmed:26725876] |
| 8 | A?Test?in?Context:?Neprilysin:?Function,?Inhibition, and?Biomarker. [pubmed:27491909] |
| European?Journal of?Heart?Failure | Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study [Pubmed: 30520545] |
| European?Journal of?Heart?Failure | Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failure [Pubmed: 30828920] |
| Clinical Research in Cardiology | Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD) [Pubmed: 32002632] |
| Neurotoxicology | Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice [Pubmed: 32522471] |
| European Heart Journal | P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction [] |
| Clin Biochem | Finding a reliable assay for soluble neprilysin [Pubmed:35331754] |
| Scientific Reports | The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in?… [] |
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