腫瘤壞死因子受體超家族成員12A(TNFRSF12A)活性蛋白
Active Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
CD266; TNFRSF12-A; FN14; TWEAKR; Tumour Necrosis Factor Related Weak Inducer Of Apoptosis; Fibroblast growth factor-inducible immediate-early response protein 14
- 編號UAPA813Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 80%
- 等電點6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant TNFRSF12A, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA813Hu01 | 腫瘤壞死因子受體超家族成員12A(TNFRSF12A)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA813Hu01 | 腫瘤壞死因子受體超家族成員12A(TNFRSF12A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA813Hu01 | 腫瘤壞死因子受體超家族成員12A(TNFRSF12A)多克隆抗體 | IHC |
| URAA813Hu21 | 腫瘤壞死因子受體超家族成員12A(TNFRSF12A)重組抗體 | FCM |
| UMAA813Hu21 | 腫瘤壞死因子受體超家族成員12A(TNFRSF12A)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA813Hu | 腫瘤壞死因子受體超家族成員12A(TNFRSF12A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA813Hu | 腫瘤壞死因子受體超家族成員12A(TNFRSF12A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Analytical Biochemistry | A single molecule assay for ultrasensitive detection of Fn14 in human serum [Pubmed: 31580829] |
