轉(zhuǎn)化生長因子β2(TGFb2)活性蛋白
Active Transforming Growth Factor Beta 2 (TGFb2)
TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor
- 編號UAPA218Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點8.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Transforming Growth Factor Beta 2 (TGFB2) belongs to TGF-beta family. As a secreted protein and a cytokine, TGFB2 plays a role in the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. Besides, TGFB2 regulates cell proliferation, differentiation, adhesion, migration by transducing the signal through transmembrane receptors type I and type II (TGFBR1 and TGFBR2). To test the effect of TGFb2 on inhibit IL4-dependent proliferation, CTLL-2 cells were seeded into triplicate wells of 96-well plates, the medium was 2% serum RPMI-1640?including various concentrations of recombinant human TGFb2. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured. Cell viability was assessed by CCK-8 assay. The result was shown in Figure1. It was obvious that TGFB2 significantly decreased cell viability of CTLL-2 cells. The ED50 of recombinant human TGFB2 is 1.95μg/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA218Hu01 | 轉(zhuǎn)化生長因子β2(TGFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA218Hu01 | 轉(zhuǎn)化生長因子β2(TGFb2)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA218Hu61 | 轉(zhuǎn)化生長因子β2(TGFb2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA218Hu01 | 轉(zhuǎn)化生長因子β2(TGFb2)多克隆抗體 | IHC; FCM |
| URAA218Hu21 | 轉(zhuǎn)化生長因子β2(TGFb2)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA218Hu21 | 轉(zhuǎn)化生長因子β2(TGFb2)單克隆抗體 | WB |
| UMAA218Hu22 | 轉(zhuǎn)化生長因子β2(TGFb2)單克隆抗體 | WB; IHC; ICC; IP. |
| UFAA218Hu08 | 抗轉(zhuǎn)化生長因子β2(TGFb2)單克隆抗體 | FCM |
| UFAA218Hu06 | 抗轉(zhuǎn)化生長因子β2(TGFb2)單克隆抗體 | FCM |
| UFAA218Hu04 | 抗轉(zhuǎn)化生長因子β2(TGFb2)單克隆抗體 | FCM |
| UFAA218Hu02 | 抗轉(zhuǎn)化生長因子β2(TGFb2)單克隆抗體 | FCM |
| USEA218Hu | 轉(zhuǎn)化生長因子β2(TGFb2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA218Hu | 轉(zhuǎn)化生長因子β2(TGFb2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA218Hu | 轉(zhuǎn)化生長因子β2(TGFb2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
| International Journal of Chemical Studies | Studies on quality attributes of skimmed colostrum powder [P-ISSN: 2349–8528] |
| JCI Insight | Angiocrine signals regulate quiescence and therapy resistance in bone metastasis [Pubmed: 31292293] |
| Mol Med | Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma [34362313] |
| Food Chem | Effect of convection and microwave heating on the retention of bioactive components in human milk [34896952] |
