轉(zhuǎn)化生長(zhǎng)因子α(TGFa)活性蛋白
Active Transforming Growth Factor Alpha (TGFa)
ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF
- 編號(hào)UAPA123Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 540 ¥ 1350 ¥ 2700 ¥ 8100 ¥ 20250
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant TGFa, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Transforming growth factor alpha?(TGF-α),?a?ligand?for the?epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. To test the effect of TGF-α on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM?prior to the addition of various concentrations of TGF-α. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of 3T3 cells after incubation with TGF-α for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of TGF-α was shown in Figure 2. It was obvious that TGF-α significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 0.6198 to 8.210ng/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Physiology and Biochemistry | Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w] |
| Oncology Reports | A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitro [PubMed: PMC3583590] |
| Sci Total Environ. | The?effects?of?particulate matter?on?inflammation?of?respiratory system:?Differences?betweenmale?and?female. [pubmed:28174048] |
| International Journal of Biological Sciences | CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 [] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets [Pubmed: 33164313] |
| Toll-like receptor 5-mediated signaling enhances liver regeneration in mice [] |
