S-期激酶關(guān)聯(lián)蛋白1(SKP1)活性蛋白
Active S-Phase Kinase Associated Protein 1 (SKP1)
SKP1A; EMC19; OCP-II; OCP2; TCEB1L; p19A; Cyclin-A/CDK2-associated p19; RNA polymerase II elongation factor-like protein; Transcription elongation factor B polypeptide 1-like
- 編號UAPF883Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點4.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
S-Phase Kinase Associated Protein 1 (SKP1) is a core component of the SKP1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. It is an adaptor protein, connecting cullin and F-box proteins in the complex. SKP1 mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Previous studies have shown that SKP1 protein is associated with Mitochondrial Dna Depletion Syndrome 13 and Parkinson. To test the effect of SKP1 on cell proliferation, A549 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human SKP1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human SKP1 for 72h. The result was shown in Figure 1. It was obvious that SKP1 significantly decreased cell viability of A549 cells. The ED50 of recombinant human SKP1 is 0.375 μg/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPF883Hu01 | S-期激酶關(guān)聯(lián)蛋白1(SKP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPF883Hu01 | S-期激酶關(guān)聯(lián)蛋白1(SKP1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAF883Hu01 | S-期激酶關(guān)聯(lián)蛋白1(SKP1)多克隆抗體 | WB,IHC,ICC/IF |
| UFAF883Hu01 | 抗S-期激酶關(guān)聯(lián)蛋白1(SKP1)多克隆抗體 | FCM |
| UMAF883Hu21 | S-期激酶關(guān)聯(lián)蛋白1(SKP1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEF883Hu | S-期激酶關(guān)聯(lián)蛋白1(SKP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMF883Hu | S-期激酶關(guān)聯(lián)蛋白1(SKP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
