P選擇素(SELP)活性蛋白
Active P-Selectin (SELP)
CD62P; Selectin, Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane
- 編號(hào)UAPA569Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 480 ¥ 1200 ¥ 2400 ¥ 7200 ¥ 18000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Selectin (SELP), a Ca(2 )-dependent receptor on myeloid cells,?belongs to the selectin/LECAM family. SELP binds to neutrophils and monocytes via?carbohydrates, it interacts with SELPLG to enable rapid leukocyte rolling over vascular surfaces in early inflammation. It has been reported that that CD24 is a ligand for P-selectin and the CD24/P-selectin binding pathway could be important in the dissemination of tumor cells by facilitating the interaction with platelets or endothelial cells. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SELP and recombinant human CD24. Briefly, biotin-linked SELP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD24-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SELP and recombinant human CD24 was shown in Figure 1, the EC50?for this effect is 0.56 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA569Hu02 | P選擇素(SELP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA569Hu61 | P選擇素(SELP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA569Hu61 | P選擇素(SELP)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA569Hu03 | P選擇素(SELP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA569Hu01 | P選擇素(SELP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA569Hu01 | P選擇素(SELP)多克隆抗體 | WB,ICC/IF |
| UPAA569Hu02 | P選擇素(SELP)多克隆抗體 | IHC |
| UFAA569Hu01 | 抗P選擇素(SELP)多克隆抗體 | FCM |
| ULAA569Hu71 | P選擇素(SELP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA569Hu81 | P選擇素(SELP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA569Hu24 | P選擇素(SELP)單克隆抗體 | WB |
| UMAA569Hu21 | P選擇素(SELP)單克隆抗體 | WB |
| UMAA569Hu22 | P選擇素(SELP)單克隆抗體 | IHC |
| UMAA569Hu23 | P選擇素(SELP)單克隆抗體 | IHC |
| USEA569Hu | P選擇素(SELP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA569Hu | P選擇素(SELP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA569Hu | P選擇素(SELP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journalof Vascular Research | Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators [Karger: 000257340] |
| Basic Research in Cardiology | Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170] |
| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
| Materials Science and Engineering: C | MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption [Pubmed:25063132] |
| Blood Coagul Fibrinolysis.? | Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. [Pubmed:25325345] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
| Endocrine | Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998] |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275] |
| Nutrition?&?Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835] |
| Available at SSRN 3335365? | Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers [] |
| Ecotoxicology?and?Environmental?Safety | Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P [] |
| Thrombosis Research | Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449] |
| JOURNAL OF CONTROLLED RELEASE | Polycaprolactone vascular graft with epigallocatechin gallate embedded sandwiched layer-by-layer functionalization for enhanced antithrombogenicity and anti … [Pubmed: 31982435] |
| OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice [Pubmed: 32377309] |
| blood advances | The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis [34559203] |
| Vaccine | The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388] |
| Int J Biol Macromol | The inhibition of TRIM35-mediated TIGAR ubiquitination enhances mitochondrial fusion and alleviates renal ischemia-reperfusion injury [Pubmed:35421414] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
