白介素2受體α(IL2Ra)活性蛋白
Active Interleukin 2 Receptor Alpha (IL2Ra)
CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen
- 編號(hào)UAPB837Mu02
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant IL2Ra, Mouse.
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
IL2RA (Interleukin-2 receptor alpha chain ), also known as Tac antigen and as CD25, was initially identified as a 55kDa membrane glycoprotein which is capable of binding IL 2.?Besides, mouse IL2 shares 75.1% AA sequence identity with rat IL2, suggesting the exist of cross-species activity. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant rat IL2RA with recombinant mouse IL2. Briefly, IL2RA were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL2RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL2RA with IL2 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPB837Mu02 | 白介素2受體α(IL2Ra)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB837Mu01 | 白介素2受體α(IL2Ra)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB837Mu02 | 白介素2受體α(IL2Ra)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB837Mu01 | 白介素2受體α(IL2Ra)多克隆抗體 | WB; IHC |
| UPAB837Mu02 | 白介素2受體α(IL2Ra)多克隆抗體 | WB; IHC; ICC; IP. |
| UFAB837Mu01 | 抗白介素2受體α(IL2Ra)多克隆抗體 | Flow cytometry. |
| UFAB837Mu41 | 抗白介素2受體α(IL2Ra)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
| UFAB837Mu51 | 抗白介素2受體α(IL2Ra)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | Flow cytometry. |
| UFAB837Mu81 | 抗白介素2受體α(IL2Ra)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
| USEB837Mu | 白介素2受體α(IL2Ra)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB837Mu | 白介素2受體α(IL2Ra)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573] |
