肝素結(jié)合性表皮生長因子(HBEGF)活性蛋白
Active Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)
HB-EGF; DTR; DTS; DTSF; HEGFL; Diphtheria Toxin Receptor; Proheparin-binding EGF-like growth factor; Diphtheria toxin receptor
- 編號(hào)UAPB479Hu62
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點(diǎn)9.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Heparin-binding Epidermal Growth Factor-like Growth Factor (HBEGF) is a member of the epidermal growth factor (EGF) family. As a ligand for the EGF receptor (EGFR), HBEGF can bind to both 170 kDa EGFR and 180 kDa ErbB4, and through heterodimerization, ErbB2. HBEGF protein involves in several processes, including epidermal growth factor receptor signaling pathway，phosphatidylinositol 3-kinase/protein kinase B signal transduction and wound healing. To test the effect of HBEGF on cell proliferation, BALB/3T3 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human HBEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human HBEGF for 72h. The result was shown in Figure 1. It was obvious that HBEGF significantly increased cell viability of BALB/3T3 cells. The ED50 of recombinant human HBEGF is 1.033μg/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPB479Hu01 | 肝素結(jié)合性表皮生長因子(HBEGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPB479Hu61 | 肝素結(jié)合性表皮生長因子(HBEGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPB479Hu62 | 肝素結(jié)合性表皮生長因子(HBEGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB479Hu62 | 肝素結(jié)合性表皮生長因子(HBEGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPB479Hu61 | 肝素結(jié)合性表皮生長因子(HBEGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB479Hu01 | 肝素結(jié)合性表皮生長因子(HBEGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB479Hu01 | 肝素結(jié)合性表皮生長因子(HBEGF)多克隆抗體 | WB; IHC |
| ULAB479Hu71 | 肝素結(jié)合性表皮生長因子(HBEGF)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAB479Hu22 | 肝素結(jié)合性表皮生長因子(HBEGF)單克隆抗體 | WB. |
| UMAB479Hu25 | 肝素結(jié)合性表皮生長因子(HBEGF)單克隆抗體 | ICC/IF |
| UMAB479Hu21 | 肝素結(jié)合性表皮生長因子(HBEGF)單克隆抗體 | IHC |
| UMAB479Hu23 | 肝素結(jié)合性表皮生長因子(HBEGF)單克隆抗體 | IHC |
| UMAB479Hu24 | 肝素結(jié)合性表皮生長因子(HBEGF)單克隆抗體 | IHC |
| USEB479Hu | 肝素結(jié)合性表皮生長因子(HBEGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB479Hu | 肝素結(jié)合性表皮生長因子(HBEGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB479Hu | 肝素結(jié)合性表皮生長因子(HBEGF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Neuropathology | Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling. [PubMed: 21118399] |
| Toxicological Sciences | Cypermethrin Induces Astrocyte Apoptosis by the Disruption of the Autocrine/Paracrine Mode of Epidermal Growth Factor Receptor Signaling [Pubmed: source] |
| PAIN RESEARCH | 軽度炎癥性膀胱痛マウスモデルの構(gòu)築とNGFの関與 [Jstage:Source] |
| European Journal of Pain | A?novel?method?for?assessing?bladder-related?pain?reveals?the?involvement?of?nerve?growth?factor?in?painassociated?with?cyclophosphamide-induced?chronic?cystitis?in?mice. [Pubmed:25820250] |
| Kinki daigaku | 2 型糖尿病モデルラットにおける糖尿病性腎癥の進(jìn)行とHB-EGF の関連性 [17844] |
| laboratory investigation | Heparin-binding epidermal growth factor contributes to COPD disease severity by modulating airway fibrosis and pulmonary epithelial–mesenchymal transition [Pubmed:29581578] |
| International Journal of Biological Sciences | CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 [] |
| Redox?Biology | Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth [Pubmed: 30769285] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets [Pubmed: 33164313] |
| 象牙芽細(xì)胞の枯渇は象牙芽細(xì)胞分化と象牙質(zhì)形成を誘導(dǎo)する [] |
