谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)活性蛋白
Active Glutathione S Transferase Alpha 3 (GSTa3)
GST-A3; GSTA3-3; GTA3; GST class-alpha member 3
- 編號(hào)UAPA671Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)9.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from?Reactive Oxygen Species?and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant rat GSTa3, mix gently. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant rat GSTa3 is >37000 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA671Ra01 | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA671Ra01 | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA671Ra01 | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)多克隆抗體 | WB; IHC |
| ULAA671Ra71 | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)多克隆抗體(生物素標(biāo)記) | WB |
| ULAA671Ra81 | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA671Ra21 | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA671Ra | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA671Ra | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA671Ra01 | 谷胱甘肽S轉(zhuǎn)移酶α3(GSTa3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Toxicon | The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the??- [33581172] |
