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M2-型丙酮酸激酶(PKM2)多克隆抗體

Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle

編號(hào)UPAA588Hu03
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA588Hu03-M2-型丙酮酸激酶(PKM2)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度0.5mg/mL
且適物種Mus musculus (Mouse，小鼠)
應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Lung Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human PKM2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Primary Ab: 0.2μg/ml Rabbit Anti-Human PKM2 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: K562 cell lysate; Lane4: MCF7 cell lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human PKM2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對PKM2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別PKM2。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Cancer Research	Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3 [AACR: 70189]
Molecular BioSystems	Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer [Pubmed:25483567]
Tumour Biol	M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells [PubMed: 26404132]
Oncotarget	Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis [Pubmed:29707118]
Journal of?Neurotrauma	Acteoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injury [Pubmed: 30318996]
Cell Reports	Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway [Pubmed: 32049010]
	Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) []
Cancer Cell International	Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
Cancer Cell Int	Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
Life (Basel)	Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib [34947916]

留言咨詢

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA588Hu01	M2-型丙酮酸激酶(PKM2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA588Hu02	M2-型丙酮酸激酶(PKM2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA588Hu03	M2-型丙酮酸激酶(PKM2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA588Hu02	M2-型丙酮酸激酶(PKM2)活性蛋白	Cell?culture;?Activity?Assays.
UPAA588Hu02	M2-型丙酮酸激酶(PKM2)多克隆抗體	WB; IHC; ICC/IF; FCM
UPAA588Hu03	M2-型丙酮酸激酶(PKM2)多克隆抗體	WB; IHC; ICC/IF
UPAA588Hu01	M2-型丙酮酸激酶(PKM2)多克隆抗體	WB; IHC; ICC/IF; FCM
UFAA588Hu01	抗M2-型丙酮酸激酶(PKM2)多克隆抗體	FCM
UFAA588Hu03	抗M2-型丙酮酸激酶(PKM2)多克隆抗體	FCM
UMAA588Hu24	M2-型丙酮酸激酶(PKM2)單克隆抗體	WB
UMAA588Hu22	M2-型丙酮酸激酶(PKM2)單克隆抗體	WB
UMAA588Hu23	M2-型丙酮酸激酶(PKM2)單克隆抗體	WB; ICC/IF
UMAA588Hu21	M2-型丙酮酸激酶(PKM2)單克隆抗體	WB
USEA588Hu	M2-型丙酮酸激酶(PKM2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA588Hu	M2-型丙酮酸激酶(PKM2)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.