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60kD熱休克蛋白(Hsp60)多克隆抗體

Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

編號(hào)UPAA822Hu02
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來(lái)源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原 RPA822Hu02-60kD熱休克蛋白(Hsp60)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度500μg/mL
且適物種Rattus norvegicus (Rat，大鼠)
應(yīng)用WB; IHC; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Primary Ab: 0.01μg/ml Rabbit Anti-Human Hsp60 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: A549 cell lysate;
Primary Ab: 0.01μg/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HSPD1 Polyclonal Antibody (Catalog # PAA822Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Human Hela cell was stained with 20μg/ml rabbit Anti-human Hsp60 Polyclonal Antibody (Catalog PAA822Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)Hsp60的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別Hsp60。

用法

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA822Hu02	60kD熱休克蛋白(Hsp60)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA822Hu01	60kD熱休克蛋白(Hsp60)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA822Hu01	60kD熱休克蛋白(Hsp60)多克隆抗體	WB; IHC; ICC/IF
UPAA822Hu02	60kD熱休克蛋白(Hsp60)多克隆抗體	WB; IHC; FCM
UFAA822Hu01	抗60kD熱休克蛋白(Hsp60)多克隆抗體	FCM
ULAA822Hu81	60kD熱休克蛋白(Hsp60)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
ULAA822Hu71	60kD熱休克蛋白(Hsp60)多克隆抗體(生物素標(biāo)記)	WB
UFAA822Hu41	抗60kD熱休克蛋白(Hsp60)多克隆抗體(藻紅素標(biāo)記)	FCM
UMAA822Hu28	60kD熱休克蛋白(Hsp60)單克隆抗體	ICC/IF
UMAA822Hu22	60kD熱休克蛋白(Hsp60)單克隆抗體	WB; IHC; ICC/IF
UMAA822Hu21	60kD熱休克蛋白(Hsp60)單克隆抗體	WB; IHC; ICC/IF
UMAA822Hu23	60kD熱休克蛋白(Hsp60)單克隆抗體	WB; IHC
UMAA822Hu24	60kD熱休克蛋白(Hsp60)單克隆抗體	WB; ICC/IF
UMAA822Hu25	60kD熱休克蛋白(Hsp60)單克隆抗體	WB
UMAA822Hu27	60kD熱休克蛋白(Hsp60)單克隆抗體	WB
UMAA822Hu26	60kD熱休克蛋白(Hsp60)單克隆抗體	WB
UFAA822Hu02	抗60kD熱休克蛋白(Hsp60)單克隆抗體	FCM
USEA822Hu	60kD熱休克蛋白(Hsp60)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UHEA822Hu	60kD熱休克蛋白(Hsp60)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA822Hu	60kD熱休克蛋白(Hsp60)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA822Hu01	60kD熱休克蛋白(Hsp60)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It(ELISA Kit) Yourself"

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Sci Rep	Hsp60 response in experimental and human temporal lobe epilepsy [PubMed: 25801186]
Cellular?and Molecular Gastroenterology and Hepatology	Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro [Pubmed: 30878663]
Breast Cancer Res Treat	Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer [Pubmed: 32601970]

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