S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體
Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)
MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8
- 編號(hào)UMAB792Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C8
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPB792Hu01-S100鈣結(jié)合蛋白A8(S100A8)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 288 ¥ 672 ¥ 960 ¥ 2400 ¥ 9600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Lymph node Tissue; Primary Ab: 20μg/ml Mouse Anti-Human S100A8 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Prostate Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Renal cancer Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lymph node Tissue
Primary Ab: 20μg/ml Mouse Anti-Human S100A8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20μg/ml Mouse Anti-Human S100A8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 30μg/ml Mouse Anti-Human S100A8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Human Leukocyte lysate; Lane2: Human Lymphocyte lysate; Lane3: Human Saliva Primary Ab: 0.5μg/ml Mouse Anti-Human S100A8 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)S100A8的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別S100A8。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPB792Hu01 | S100鈣結(jié)合蛋白A8(S100A8)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB792Hu02 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB792Hu01 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB792Hu01 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | WB; IHC |
| UPAB792Hu02 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | IHC; FCM |
| ULAB792Hu81 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAB792Hu71 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAB792Hu21 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB; IHC |
| UMAB792Hu25 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | IHC; ICC/IF |
| UMAB792Hu22 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB; IHC |
| UMAB792Hu24 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB; ICC/IF |
| UMAB792Hu27 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | ICC/IF |
| UMAB792Hu29 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | ICC/IF |
| UMAB792Hu26 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB |
| UMAB792Hu28 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB |
| UMAB792Hu23 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC/IF |
| UFAB792Hu02 | 抗S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | FCM |
| UFAB792Hu04 | 抗S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | FCM |
| UFAB792Hu06 | 抗S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | FCM |
| USEB792Hu | S100鈣結(jié)合蛋白A8(S100A8)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB792Hu | S100鈣結(jié)合蛋白A8(S100A8)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSB792Hu01 | S100鈣結(jié)合蛋白A8(S100A8)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSB792Hu01 | S100鈣結(jié)合蛋白A8(S100A8)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biochemical and Biophysical Research Communications | High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X] |
| American Journal of Reproductive Immunology | Association of Increased S100A8 Serum Protein with Early Pregnancy Loss [Pubmed:25252120] |
| the faseb journal | Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702] |
| J Immunol | S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet [PubMed: 26608915] |
| PLoS?One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680] |
| Journal?of?Alzheimer's?Disease | Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology. [pubmed:27911324] |
| ?Journal of Functional Foods | Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208] |
| International?Journal?of?Molecular?Medicine | Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells [pubmed:28498464] |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877] |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3] |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5] |
| Journal of Biological Chemistry | Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993] |
| European?review for?medical?and pharmacological?sciences | S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169] |
| Foodborne Pathogens and Disease | Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394] |
| Kidney International Reports | Systemic Inflammation Precedes Microalbuminuria in Diabetes [] |
| Cancer Management and Research | iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia [Pubmed: 31440093] |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598] |
| ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE | Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis [] |
| MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227] |
| university of copenhagen | Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in [] |
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