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組蛋白脫乙?；?(HDAC1)單克隆抗體

Monoclonal Antibody to Histone Deacetylase 1 (HDAC1)

HDAC1; GON10; HD1; RPD3; RPD3L1

酶與激酶

編號(hào)UMAC206Hu23
物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來(lái)源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))E31
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原 RPC206Hu01-組蛋白脫乙?；?(HDAC1)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種-
應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格 20μl100μl 200μl 1ml 10ml
價(jià)格 ¥ 344 802 ¥ 802 ¥ 1146 ¥ 2865 ¥ 11460
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Stomach Tissue; Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Stomach cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Western Blot; Sample: Lane1: Human Lung lysate; Lane2: A549 cell lysate; Lane3: HepG2 cell lysate Primary Ab: 0.2μg/ml Mouse Anti-Human HDAC1 Antibody Second Ab: 0.2μg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Human Jurkat cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human HDAC1 Monoclonal Antibody (Catalog MAC206Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)HDAC1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別HDAC1。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-30μg/mL;
Flow cytometry: 10μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Journal of Integrative Medicine	Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase [Pubmed:25609369]
Tzu Chi Medical Journal	Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cells [Science: Article]
Restor Neurol Neurosci	Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal rats [PubMed: 26410212]
Pharmacol Biochem Behav	Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's disease [PubMed: 25931268]
網(wǎng)頁(yè)打不開(kāi)	Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cells [S1016319016300295]

留言咨詢(xún)

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
UAPC206Hu01	組蛋白脫乙?；?(HDAC1)活性蛋白	Cell?culture;?Activity?Assays.
URPC206Hu01	組蛋白脫乙?；?(HDAC1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAC206Hu01	組蛋白脫乙酰基酶1(HDAC1)多克隆抗體	WB; ICC/IF
UMAC206Hu23	組蛋白脫乙?；?(HDAC1)單克隆抗體	WB; IHC; ICC/IF; FCM
UMAC206Hu24	組蛋白脫乙?；?(HDAC1)單克隆抗體	WB; IHC; FCM
UMAC206Hu25	組蛋白脫乙?；?(HDAC1)單克隆抗體	WB; IHC
UMAC206Hu26	組蛋白脫乙?；?(HDAC1)單克隆抗體	WB; IHC
UMAC206Hu27	組蛋白脫乙?；?(HDAC1)單克隆抗體	WB; IHC; ICC/IF
UMAC206Hu28	組蛋白脫乙酰基酶1(HDAC1)單克隆抗體	WB
UMAC206Hu29	組蛋白脫乙?；?(HDAC1)單克隆抗體	IHC
UMAC206Hu22	組蛋白脫乙?；?(HDAC1)單克隆抗體	WB; IHC; ICC/IF; FCM
UMAC206Hu21	組蛋白脫乙?；?(HDAC1)單克隆抗體	WB; IHC; ICC/IF
UFAC206Hu02	抗組蛋白脫乙酰基酶1(HDAC1)單克隆抗體	FCM
UFAC206Hu08	抗組蛋白脫乙?；?(HDAC1)單克隆抗體	FCM
UFAC206Hu06	抗組蛋白脫乙?；?(HDAC1)單克隆抗體	FCM
UFAC206Hu04	抗組蛋白脫乙?；?(HDAC1)單克隆抗體	FCM
USEC206Hu	組蛋白脫乙?；?(HDAC1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMC206Hu	組蛋白脫乙?；?(HDAC1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UPGC206Hu01	組蛋白脫乙?；?(HDAC1)引物對(duì)	PCR

組蛋白脫乙?；?(HDAC1)單克隆抗體