雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體
Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1)
DCDC3A; DCLK; DCAMKL1; Doublecortin And CaM Kinase-Like 1; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1
- 編號UMAF390Hu23
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C1
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPF390Hu01-雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 344 ¥ 802 ¥ 1146 ¥ 2865 ¥ 11460
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Cerebellum Tissue; Primary Ab: 30μg/ml Mouse Anti-Human DCLK1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2μg/ml Mouse Anti-Human DCLK1 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human DCLK1 Monoclonal Antibody (Catalog MAF390Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對DCLK1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別DCLK1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPF390Hu01 | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAF390Hu01 | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)多克隆抗體 | WB; IHC |
| UMAF390Hu22 | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | WB; IHC |
| UMAF390Hu23 | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | WB; IHC; FCM |
| UMAF390Hu24 | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | WB |
| UMAF390Hu25 | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | IHC |
| UMAF390Hu21 | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | WB; IHC |
| UFAF390Hu04 | 抗雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | Flow cytometry. |
| UFAF390Hu02 | 抗雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | Flow cytometry. |
| UFAF390Hu08 | 抗雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | Flow cytometry. |
| UFAF390Hu06 | 抗雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)單克隆抗體 | Flow cytometry. |
| USEF390Hu | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMF390Hu | 雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One | Doublecortin-Like Kinase 1 Is Elevated Serologically in Pancreatic Ductal Adenocarcinoma and Widely Expressed on Circulating Tumor Cells [PubMed: 25723399] |
| Biomarker Research | Elevated doublecortin-like kinase 1 serum levels revert to baseline after therapy in early stage esophageal adenocarcinoma [] |
| Journal of Retina-Vitreous | Serum Doublecortin-like Kinase-1 Levels in Age-Related Macular Degeneration. [] |
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