乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Lactate Dehydrogenase (LDH)
- 編號UMEB864Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍3.12-200ng/mL
- 靈敏度最小可檢測劑量小于等于1.14ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1676 ¥ 2394 ¥ 10773 ¥ 20349 ¥ 167580
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-101 | 91 |
| EDTA plasma(n=5) | 86-104 | 91 |
| heparin plasma(n=5) | 86-102 | 98 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-96% | 83-98% | 86-93% | 84-92% |
| EDTA plasma(n=5) | 78-96% | 97-105% | 94-101% | 98-105% |
| heparin plasma(n=5) | 96-105% | 82-97% | 98-105% | 83-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL，37°C溫育1小時；
3. 吸棄，加檢測溶液A25µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數(shù)。
實驗原理
將乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)與連接于固相載體上的抗體結合，然后加入生物素化的乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UPAB864Hu01 | 乳酸脫氫酶(LDH)多克隆抗體 | Testing in progress |
| ULAB864Hu71 | 乳酸脫氫酶(LDH)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAB864Hu22 | 乳酸脫氫酶(LDH)單克隆抗體 | WB; IHC; ICC; IP. |
| USEB864Hu | 乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEB864Hu | 乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB864Hu | 乳酸脫氫酶(LDH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Neuroscience Research | Hydrogen sulfide antagonizes homocysteine-induced neurotoxicity in PC12 cells [PubMed: 20674619] |
| Clinical and Experimental Pharmacology and Physiology | Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity [PubMed: 21261675] |
| Neurochemistry International | Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential [ScienceDirect: S0197018612001428] |
| ostaglandins Leukot Essent Fatty Acids. | Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. [Pubmed: 24095588] |
| Journal of Diabetes and its Complications | Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor [Pubmed:25650182] |
| Cancer Science & Therapy | An Additional risk of Lung Cancer from Recurrent Exposure to Ethyl Carbamate (EC) in BALB/C Mice [Open-Access: 1000375] |
| Journal of Antimicrobial Chemotherapy | NLF20: an antimicrobial peptide with therapeutic potential against invasive Pseudomonas aeruginosa infection [PubMed: 26503666] |
| American Journal of Physology Heart and Circulatory Physiology | Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice [Pubmed:27288439] |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385] |
| Experimental Cell Research | UpreCavia (Guinea pig )lation of CBS/H2S system contributes to asymmetric dimethylarginine-triggered protection against the neurotoxicity of glutamate to PC12 cells by inhibiting NOS/NO pathway [science:S0014482716301598] |
| Blood Cells Mol Dis | Transgenic sickle cell trait mice do not exhibit abnormal thermoreCavia (Guinea pig )latory and stress responses to heat shock exposure [Pubmed:27282581] |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. [pubmed:27907155] |
| Scientific Reports | Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway. [pubmed:27678302] |
| Experimental Biology and Medicine | Ameliorating effect of anti-Alzheimer’s drugs on the bidirectional association between type 2 diabetes mellitus and Alzheimer’s disease [pubmed:28534431] |
| Cancer?Science | A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme?… [Scholarena:Source] |
| Molecular Medicine Reports | Protective effects of N (2)?L?alanyl?L?glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473] |
| Molecular?Medicine?Reports? | Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781] |
| Oncology?Letters | A novel potential effective strategy for enhancing the antitumor immune response in breast cancer patients using a viable cancer cell?dendritic cell?based vaccine [Pubmed:29928442] |
| Experimental Allergy and Immunology | Osteopontin protects against pneumococcal infection in a murine model of allergic airway inflammation [Pubmed: 30362569] |
| Scientific?Reports | Low plasma haptoglobin is a risk factor for life-threatening childhood severe malarial anemia and not an exclusive consequence of hemolysis [Pubmed: 30510258] |
| Molecular Medicine Reports | Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury [Pubmed: 32705277] |
| Cell Transplant | Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells Protect Cardiac Cells Against Hypoxia/Reoxygenation Injury by Inhibiting?… [Pubmed: 32864999] |
| Nanomaterials | Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313] |
| Journal of Renal Injury Prevention | Prevention of kidney injury after myocardial ischemia reperfusion is achievable with short-term protein restriction [] |
| clinical and translational medicine | Milk fat globule EGF factor 8 restores mitochondrial function via integrin?\medicated activation of the FAK?\STAT3 signaling pathway in acute pancreatitis [33634976] |
| Biomed Res Int | Factors Affecting the Incidence, Progression, and Severity of COVID-19 in Type 1 Diabetes Mellitus [34840966] |
| Cell Cycle | Improvement of autophagic flux mediates the protection of hydrogen sulfide against arecoline-elicited neurotoxicity in PC12 cells [Pubmed:35316162] |
| Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating?… [Pubmed:35281603] |
| Int Immunopharmacol | LncRNA KCNQ1OT1 attenuates myocardial injury induced by hip fracture via regulating of miR-224-3p/GATA4 axis [Pubmed:35217336] |
