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催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay)

LTH; Luteotropic Hormone

（注：單次混測多因子不超過8個指標）

編號ULMA846Rb
物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法競爭抑制
反應(yīng)時長1.5h
檢測范圍0.2-200ng/mL
靈敏度最小可檢測劑量小于等于0.067 ng/mL.
樣本類型Serum, plasma and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	94-101	97
EDTA plasma(n=5)	78-91	82
heparin plasma(n=5)	92-99	95
sodium citrate plasma(n=5)	90-102	96

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	89-96%	86-101%	89-101%	90-97%
EDTA plasma(n=5)	94-101%	89-102%	80-93%	85-99%
heparin plasma(n=5)	98-105%	90-101%	83-98%	85-95%
sodium citrate plasma(n=5)	94-102%	84-95%	95-103%	90-99%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。

贈品

增值服務(wù)

實時熒光定量PCR實驗服務(wù)

參考文獻

雜志	參考文獻
The Journal of Biological Chemistry	In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland [Jcb: 39297]
Journal of Clinical Neuroscience	TLR9 expression is associated with prognosis in patients with glioblastoma multiforme [ScienceDirect: S0967586811003572]
Sensors and Actuators B: Chemical	Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes [Sciencedirect:S0925400514000720]
Veterinary Word	Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
Journal?of?Dairy?Science	Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
Stem Cell Research & Therapy	Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells [pubmed:29096715]
Stem?Cell?Research?&?Therapy	The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657]
Frontiers in Molecular Neuroscience	Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
Toxicology and?Applied?Pharmacology	The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance [Pubmed:29551354]
Journal of Cellular Biochemistry	The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway [Pubmed:29388711]
Journal?of?Cellular?and?Molecular?Medicine	Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenografts [Pubmed: 30334324]
BMC?Veterinary Research	Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome [Pubmed: 30404636]
International Journal of Basic and Clinical Endocrinology	Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940]
MORPHOLOGY AND PATHOMORPHOLOGY	Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment [Pubmed: 32152847]
JOURNAL OF DAIRY SCIENCE	Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle [Pubmed: 32684470]
BioMed Research International	Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
Vet Microbiol	Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection [34391195]
Antioxidants (Basel)	Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer [34439574]
Nutrients	Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]

留言咨詢

編號	適用物種：Oryctolagus cuniculus (Rabbit，兔)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA846Rb01	催乳素(PRL)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA846Rb51	催乳素(PRL)多克隆抗體	WB; IHC; ICC; IP.
UPAA846Rb01	催乳素(PRL)多克隆抗體	WB; IHC; ICC; IP.
UMAA846Rb21	催乳素(PRL)單克隆抗體	WB; IHC; ICC; IP.
UCEA846Rb	催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA846Rb	催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.