Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Procollagen II C-Terminal Propeptide (PIICP) ,etc. by FLIA (Flow Luminescence Immunoassay)
P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide
（注：單次混測多因子不超過8個(gè)指標(biāo) ）
- 編號(hào)ULMA964Eq
- 物種Equus caballus; Equine (Horse，馬) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3.5h
- 檢測范圍3.91-4000pg/mL
- 靈敏度最小可檢測劑量小于等于1.303 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-93 | 82 |
| EDTA plasma(n=5) | 93-101 | 96 |
| heparin plasma(n=5) | 93-101 | 96 |
| sodium citrate plasma(n=5) | 92-99 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-96% | 91-98% | 97-105% | 95-102% |
| EDTA plasma(n=5) | 84-101% | 91-99% | 89-102% | 96-104% |
| heparin plasma(n=5) | 93-101% | 87-95% | 85-92% | 89-96% |
| sodium citrate plasma(n=5) | 83-103% | 90-102% | 94-103% | 90-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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| 編號(hào) | 適用物種：Equus caballus; Equine (Horse，馬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| USEA964Eq | Ⅱ型前膠原羧基端原肽(PIICP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA964Eq | Ⅱ型前膠原羧基端原肽(PIICP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA964Eq | Ⅱ型前膠原羧基端原肽(PIICP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Experimental and Therapeutic Medicine | Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: A … [10.3892] |
| Functional Foods in Health & Disease | Evaluation of the effect of administering N-acetyl-glucosamine-containing green tea supplement on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: a randomized double-blind placebo-controlled clinical study [309] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Effect of N-acetylglucosamine administration on cartilage metabolism and safety in healthy subjects without symptoms of arthritis: A case report [pubmed:28413518] |
| Experimental?and?Therapeutic?Medicine | Evaluation of the effect of salmon nasal proteoglycan on biomarkers for cartilage metabolism in individuals with knee joint discomfort: A randomized double?blind placebo?controlled clinical study [etm:14] |
| Experimental and Therapeutic Medicine | Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double?blind, placebo?controlled study [10.3892/etm.2017.5064] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Evaluation of the chondroprotective action of N?acetylglucosamine in a rat experimental osteoarthritis [pubmed:28912864] |
| Functional Foods In Health And Disease | Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals: a randomized double-blind placebo-controlled clinical study [view/366] |
| Spine | No effects of hyperosmolar culture medium on tissue regeneration by human degenerated nucleus pulposus cells despite upregulation extracellular matrix genes [fulltext:2018/03010] |
| Advances in?Clinical?and Experimental?Medicine | Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with?… [68362.pdf] |
| Functional Food Research | サケ鼻軟骨由來プロテオグリカン摂取による関節(jié)保護(hù)効果 [] |
| Spine | No Effects of Hyperosmolar Culture Medium on Tissue Regeneration by Human Degenerated [Pubmed: 25856264] |
| Applied Physiology Nutrition and Metabolism | Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trial [Pubmed: 31990581] |
| International Journal of Molecular Sciences | Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration [Pubmed: 32295299] |
| Biomolecules | Concerted Actions by PIICP, CTXII, and TNF-|á in Patients with Juvenile Idiopathic Arthritis [33924892] |
