脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Phospholipase A2 Group VII (LpPLA2) ,etc. by FLIA (Flow Luminescence Immunoassay)
PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA867Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.78-800pg/mL
- 靈敏度最小可檢測劑量小于等于0.26 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-103 | 98 |
| EDTA plasma(n=5) | 90-104 | 101 |
| heparin plasma(n=5) | 88-96 | 93 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-102% | 81-89% | 85-93% | 89-103% |
| EDTA plasma(n=5) | 91-98% | 81-90% | 84-97% | 85-98% |
| heparin plasma(n=5) | 91-99% | 85-103% | 78-93% | 80-101% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
贈品
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相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA867Ra61 | 脂蛋白關聯磷脂酶A2(LpPLA2)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA867Ra61 | 脂蛋白關聯磷脂酶A2(LpPLA2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA867Ra01 | 脂蛋白關聯磷脂酶A2(LpPLA2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA867Ra01 | 脂蛋白關聯磷脂酶A2(LpPLA2)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA867Ra | 脂蛋白關聯磷脂酶A2(LpPLA2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA867Ra | 脂蛋白關聯磷脂酶A2(LpPLA2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| China Pharmacy | Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats [Source] |
| Atherosclerosis | Everolimus therapy is associated with reduced lipoprotein-associated phospholipase A2 (Lp-Pla2) activity and oxidative stress in heart transplant recipients. [Pubmed: 23958269] |
| Journal of Diabetes Research | Lipoprotein-Associated Phospholipase A2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus [Hindawi: 278063] |
| Health | The Role of Asymmetric Dimethylarginine and Lipoprotein Associated Phospholipase A2 in Children and Adolescents with Dyslipidemia [Scirp:Source] |
| Biology Bulletin | In vitro lipid-lowering and fibrinolytic effects of regulatory leucine-containing glyprolines in human blood [Springer:Source] |
| Gynecol Endocrinol. | Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392] |
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