基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Matrix Metalloproteinase 2 (MMP2) ,etc. by FLIA (Flow Luminescence Immunoassay)
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA100Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.01-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.003 ng/mL.
- 樣本類型serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
- 價格 ¥ 1214 ¥ 1260 ¥ 1330 ¥ 1424 ¥ 1517 ¥ 1657 ¥ 1867 ¥ 2334 添加到價格計算器 計算器
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-94 | 89 |
| EDTA plasma(n=5) | 80-97 | 94 |
| heparin plasma(n=5) | 88-103 | 98 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-101% | 98-105% | 92-102% | 96-103% |
| EDTA plasma(n=5) | 88-102% | 97-104% | 88-99% | 82-89% |
| heparin plasma(n=5) | 89-99% | 94-105% | 79-92% | 78-88% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA100Mu01 | 基質(zhì)金屬蛋白酶2(MMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA100Mu01 | 基質(zhì)金屬蛋白酶2(MMP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA100Mu02 | 基質(zhì)金屬蛋白酶2(MMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA100Mu01 | 基質(zhì)金屬蛋白酶2(MMP2)多克隆抗體 | WB |
| UPAA100Mu02 | 基質(zhì)金屬蛋白酶2(MMP2)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA100Mu | 基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA100Mu | 基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA100Mu | 基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA100Mu | 基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA100Mu | 基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Moleculer Vision | Bone morphogenetic protein-2: a potential regulator in scleral remodeling [PubMed: PMC2605409] |
| Neuroimmunomodulation | Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source] |
| Zahedan Journal of Research in Medical Sciences | The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men [Zjrms: Source] |
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source] |
| Experimental & Translational Stroke Medicine | Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke [PubMed: 21679435] |
| PLoS One | Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 [PubMed: PMC3797697] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Clinical and Investigative Medicine | The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells [Pubmed: 23544611] |
| Physiol. Res. | The unwounded skin remodeling in animal models of diabetes types 1 and 2. [Pubmed: 24020818] |
| Acta Histochemica | RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells [Pubmed: 24681113] |
| Peritoneal Dialysis | Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients? [Pubmed: 23994841] |
| Polski Przeglad Chirurgiczny | Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course [Pubmed: 23585206] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Int Immunopharmacol.? | Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis [Pubmed:25824718] |
| Journal of Perinatology | Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion [Pubmed:25078863] |
| Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
| Stem Cells International | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro [Pubmed:25767513] |
| Physiology and Pharmacology | A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men [Phypha:Source] |
| Mediators of Inflammation | Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse [Journals: Mi] |
| Journal of Biomaterials and Tissue Engineering | MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 [Content: Asp] |
| Biomedical Materials | In vivo [PubMed: 25634132] |
| Int J Clin Exp Pathol | Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways [PubMed: 26191138] |
| Hattori and Ishihara Stem Cell Research & Therapy | Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells [PubMed: 25884474] |
| Biosens Bioelectron | Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+ [PubMed: 26433066] |
| Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140] |
| Canadian Journal of Physiology and Pharmacology | Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats [Doi: Abs] |
| PLASTIC AND AESTHETIC RESEARCH | A comparative study to evaluate the effect of limited access dressing on diabetic ulcers [Article: 2347-9264] |
| Int J Mol Sci | Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ [PubMed: 26703570] |
| Basic Research | CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma [Journal:27699] |
| International Journal of Oncology | 1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway [Pubmed:26782341] |
| International Journal of Pharmacology | Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration [profile:57162df608aebf0697f1843d] |
| Journal of Stroke and Cerebrovascular Diseases | Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats [Pubmed:26851971] |
| VirusDisease | Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446] |
| Cell Physiol Biochem. | MicroRNA-340 Induces?Apoptosis?and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 [Pubmed:27160777] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748] |
| Biosensors and Bioelectronics | Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates [science:S0956566315304474] |
| world journal of surgery | Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia. [pubmed:28050662] |
| disease markers | Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. [pubmed:28348451] |
| Balkan Medical Journal | Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage [pubmed:27761275] |
| Microvascular?Research | Pleiotropic?effects?of?survivin?in?vascular endothelial cells. [pubmed:27373518] |
| BMC Veterinary Research | Metalloproteinases?and their?inhibitors?are?influenced?by?inhalative?glucocorticoid?therapy?incombination?with?environmental?dust?reduction?in?equine?recurrent airway obstruction. [pubmed:27938355] |
| Cellular?Physiology?and?Biochemistry | MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. [pubmed:27160777] |
| Journal of Biomaterials and Tissue Engineering | MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line [publication:306067745] |
| World?Journal?of?Plastic?Surgery | Superficial?Burn?Wound Healing?with?Intermittent?Negative Pressure Wound Therapy?UnderLimited?Access?and?Conventional?Dressings. [pubmed:27853690] |
| Journal of Biomaterials and Tissue Engineering | Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts [art00008] |
| Human cell | Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity [pubmed:28091828] |
| Human Cell | MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 [pubmed:27743351] |
| Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422] |
| Human Cell | MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1 [pubmed:28573504] |
| Cellular Physiology and Biochemistry | The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells [pubmed:29224019] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748] |
| Fundamental & Clinical Pharmacology | Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9 [DOI:10.1111/fcp.12294] |
| PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis [pubmed:28665955] |
| Nanotoxicology | Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway [10.1080/17435390.2017.1357213] |
| Archives of Medical Science | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. [pubmed:28507585] |
| Molecular Medicine Reports | Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen?induced arthritis [pubmed:28901438] |
| European?Journal of Medicinal Chemistry | Syntenin-targeted peptide blocker inhibits progression of cancer cells [Pubmed:29857221] |
| Journal of Neurochemistry | Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling [Pubmed:29572844] |
| Journal?of?Cellular?Biochemistry | Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia?… [10.1002:jcb.26849] |
| Inflammation | Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy [Pubmed:29704151] |
| OncoTargets?and Therapy | sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion [Pubmed:30022839] |
| Biomed?Research?International | CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling [Pubmed:29984229] |
| APMIS | Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamide [Pubmed:30058214] |
| Molecular?Medicine?Reports | Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitro [Pubmed:29328476] |
| Oncology?Letters | miRNA?199a?5p suppresses proliferation and invasion by directly targeting NF?κB1 in human ovarian cancer cells [10.3892:ol.2018.9170] |
| Taiwanese Journal of Obstetrics & Gynecology | HMGB1-RAGE signaling pathway in pPROM [Pubmed:29673663] |
| Open?Medicine | MMP-2 and TIMP-2 in patients with heart failure and chronic kidney disease [Pubmed:29915813] |
| Pediatric?Research | Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. [Pubmed: 30464333] |
| Advanced?Healthcare?Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809] |
| Cellular & Molecular Biology Letters | MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1 [] |
| Mediators of Inflammation | Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma [] |
| Journal of endovascular therapy: an official journal of the International Society of Endovascular Specialists | Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular?… [Pubmed: 31464166] |
| Oncology Research | MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 [Pubmed: 31796150] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
| Acta Biomaterialia | Stiffness of the Aligned Fibers Affects Structural and Functional Integrity of the Oriented Endothelial Cells [Pubmed: 32205213] |
| JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289] |
| JOURNAL OF ETHNOPHARMACOLOGY | Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling?… [Pubmed: 32745509] |
| heliyon | Matrix levels of metalloproteinase-2 (MMP-2) and toxicity evaluation of carbonate apatite-based endodontic sealer in rat subcutaneous implantation [Pubmed: 32671254] |
| J Cell Physiol | A novel role of VEPH1 in regulating AoSMC phenotypic switching [Pubmed: 32342520] |
| Advances in Animal and Veterinary Sciences | Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats [] |
| Alteration on MMP-2 Levels in Women Diabetes Patients in Basrah [] | |
| Veterinary Sciences: Research and Reviews | Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep [] |
| MEDIATORS OF INFLAMMATION | MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic [33488296] |
| Oncol Lett | GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression [33613720] |
| Journal of Pregnancy | Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia [33381317] |
| Immunopharmacol Immunotoxicol | Tofacitinib enhances remyelination and improves myelin integrity in cuprizone-induced mice [34618622] |
| Exp Cell Res | Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human?… [34762897] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
| Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer [34650982] | |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980] |
| Journal of Pharmaceutical Research International | Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid?… [] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] | |
| Turkish Journal of Biochemistry-Turk Biyokimya Dergisi | Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes [] |
留言咨詢
