免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay)
IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA545Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.1-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.033 ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-94 | 87 |
| EDTA plasma(n=5) | 82-90 | 86 |
| heparin plasma(n=5) | 92-101 | 96 |
| sodium citrate plasma(n=5) | 84-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-93% | 84-98% | 83-97% | 90-104% |
| EDTA plasma(n=5) | 80-95% | 90-101% | 87-96% | 87-96% |
| heparin plasma(n=5) | 96-103% | 90-97% | 94-104% | 90-101% |
| sodium citrate plasma(n=5) | 84-99% | 82-101% | 79-89% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
贈品
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相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UNPA545Hu01 | 免疫球蛋白E(IgE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA545Hu61 | 免疫球蛋白E(IgE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA545Hu01 | 免疫球蛋白E(IgE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA545Hu01 | 免疫球蛋白E(IgE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA545Hu01 | 免疫球蛋白E(IgE)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA545Hu71 | 免疫球蛋白E(IgE)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| URAA545Hu21 | 免疫球蛋白E(IgE)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA545Hu22 | 免疫球蛋白E(IgE)單克隆抗體 | WB |
| UMAA545Hu21 | 免疫球蛋白E(IgE)單克隆抗體 | WB |
| UMAA545Hu23 | 免疫球蛋白E(IgE)單克隆抗體 | WB |
| USEA545Hu | 免疫球蛋白E(IgE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA545Hu | 免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA545Hu01 | 免疫球蛋白E(IgE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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