肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Hepatocyte Growth Factor (HGF) ,etc. by FLIA (Flow Luminescence Immunoassay)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
（注：單次混測多因子不超過8個(gè)指標(biāo) ）
- 編號(hào)ULMA047Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 94 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 90-103 | 96 |
| sodium citrate plasma(n=5) | 88-96 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-98% | 87-97% | 95-102% | 92-99% |
| EDTA plasma(n=5) | 84-91% | 93-101% | 97-104% | 90-101% |
| heparin plasma(n=5) | 86-101% | 88-96% | 96-104% | 84-93% |
| sodium citrate plasma(n=5) | 81-98% | 95-103% | 90-98% | 90-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA047Mu02 | 肝細(xì)胞生長因子(HGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA047Mu01 | 肝細(xì)胞生長因子(HGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA047Mu01 | 肝細(xì)胞生長因子(HGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA047Mu01 | 肝細(xì)胞生長因子(HGF)多克隆抗體 | IHC |
| UPAA047Mu02 | 肝細(xì)胞生長因子(HGF)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA047Mu | 肝細(xì)胞生長因子(HGF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA047Mu | 肝細(xì)胞生長因子(HGF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA047Mu | 肝細(xì)胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Gene Therapy and Molecular Biology | Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart [LWW: 01000] |
| Journal of Translational Medicine | Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma [BioMed: 14795876] |
| Digestive Diseases and Sciences | Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure [SpringerLink: k22823l138x2344x] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998] |
| Cell Journal (Yakhteh) | Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis [PubMed: PMC3584430] |
| Gastroenterol Res Pract | Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis [PubMed: 26448742] |
| Tumor Biology | Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Cytotherapy | Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury [PubMed: 25863963] |
| Biomedicine & Pharmacotherapy | Silymarin-loaded Eudragit? RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats [Pubmed:27261582] |
| Can J Physiol Pharmacol.? | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| Changes in HGF levels after a progressive test before and after Tabata HIIT program [profile:Pawel_Wolanski3] | |
| Stem Cell Research & Therapy | Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats [10.1186/s13287-017-0739-3] |
| Oncology?Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c?Met/PI3K/AKT in vitro [10.3892/ol.2017.7301] |
| Stem Cell Research & Therapy | Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. [pubmed:28679425] |
| Nature?Communications | Pulmonary pericytes regulate lung morphogenesis [Pubmed:29934496] |
| Experimental And Molecular?Medicine | Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke?… [Pubmed:29650950] |
| Oncology?Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro [Pubmed:29375714] |
| Bulletin?of Experimental?Biology?and?Medicine | Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats [Pubmed:29796817] |
| Cell Biology International | Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection [Pubmed: 31297922] |
| Journal of stem cells & regenerative medicine | What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate? [Pubmed: 31239605] |
| Open Journal of Veterinary Medicine | Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury [Pubmed: 32821665] |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care [33469783] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
