谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Glutathione (GSH) ,etc. by FLIA (Flow Luminescence Immunoassay)
L-Reduced Glutathione
（注：單次混測多因子不超過8個(gè)指標(biāo) ）
- 編號ULMA294Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長1.5h
- 檢測范圍0.1-100ug/mL
- 靈敏度最小可檢測劑量小于等于0.033 ug/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
- 價(jià)格 ¥ 1490 ¥ 1547 ¥ 1633 ¥ 1748 ¥ 1862 ¥ 2034 ¥ 2292 ¥ 2865 添加到價(jià)格計(jì)算器 計(jì)算器
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-99 | 85 |
| EDTA plasma(n=5) | 92-101 | 97 |
| heparin plasma(n=5) | 86-101 | 90 |
| sodium citrate plasma(n=5) | 88-102 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-103% | 92-99% | 82-94% | 89-102% |
| EDTA plasma(n=5) | 89-97% | 83-95% | 78-96% | 85-97% |
| heparin plasma(n=5) | 91-102% | 84-97% | 98-105% | 95-104% |
| sodium citrate plasma(n=5) | 80-88% | 96-103% | 79-94% | 85-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| USPA294Ge01 | 谷胱甘肽(GSH)合成多肽 | Immunogen; Blocking Peptide. |
| UCPA294Ge11 | 谷胱甘肽(GSH)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA294Ge21 | 谷胱甘肽(GSH)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA294Ge41 | 谷胱甘肽(GSH)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA294Ge01 | 谷胱甘肽(GSH)多克隆抗體 | ELISA |
| ULAA294Ge71 | 谷胱甘肽(GSH)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA294Ge22 | 谷胱甘肽(GSH)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA294Ge21 | 谷胱甘肽(GSH)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UIEA294Ge | 谷胱甘肽(GSH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA294Ge | 谷胱甘肽(GSH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA294Ge | 谷胱甘肽(GSH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA294Ge | 谷胱甘肽(GSH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA294Ge11 | 谷胱甘肽(GSH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| European Journal of Clinical Nutrition (2012) 66 | Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants [PubMed: 22781023] |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
| Reproduction | Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis [Pubmed:25376627] |
| Iran J Basic Med Sci | Effects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitation [PubMed: 25810895] |
| Bulletin of the Veterinary Institute in Pulawy | Oxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird's Diet [View: J] |
| Journal of Allergy and Clinical Immunology | Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress [Pubmed:27016803] |
| Oxidative Medicine and Cellular Longevity | Hydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathway [journals:2153745.pdf] |
| Institutional repository of Vilnius University | Rapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone induction [object:elaba:15733325] |
| Molecular Medicine Reports | Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells [pubmed:28260084] |
| Molecular?Medicine?Reports | Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells [pubmed:29115483] |
| Cancer Medicine | Metabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic mice [pubmed:28941178] |
| Free?radical?research | Circulating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma?… [Pubmed:29463155] |
| Biomed?Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336] |
| Molecular?Medicine?Reports | Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF?β1/Smad3 signaling pathway [Pubmed:29620209] |
| Experimental and Therapeutic?Medicine | Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO?1 signaling pathway [Pubmed:30116359] |
| Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002] |
| EXPERIMENTAL ANIMALS | Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation [Pubmed: 30606939] |
| Acta?Biomaterialia | Porous Se@ SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species?… [Pubmed: 30753941] |
| Molecular Medicine Reports | MicroRNA?21 contributes to the puerarin?induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury [Pubmed: 31115556] |
| Earth and Environmental Science | Germinated-soy milk as a healthy diet to induce high antioxidant enzymes in breast milk [] |
| Frontiers in Neuroscience | Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus [Pubmed: 32218719] |
| Oxidative Medicine and Cellular Longevity | Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress [Pubmed: 31827710] |
| Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2?… [Pubmed: 32300366] |
| Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β?hydroxybutyrate on kainic acid?induced epilepsy [Pubmed: 33101467] |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
| Toxicology | Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect [33259821] |
| CELLULAR AND MOLECULAR LIFE SCIENCES | Glutamine deficiency promotes stemness and chemoresistance in tumor cells through DRP1-induced mitochondrial fragmentation [33895866] |
| Exp Ther Med | Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats [34093766] |
| Experimental and Therapeutic Medicine | MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma [33732346] |
| chemosphere | Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective [33662726] |
| Molecular Medicine Reports | Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats [34278476] |
| J Healthc Eng | κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway [34608407] |
| Cell Death Discov | Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats [Pubmed:35022384] |
| J Dairy Sci | Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease [Pubmed:35086715] |
| Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice [Pubmed:35740008] |
| J Inflamm Res | The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats [Pubmed:35645576] |
留言咨詢
