堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Fibroblast Growth Factor 2, Basic (FGF2) ,etc. by FLIA (Flow Luminescence Immunoassay)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA551Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長1.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-103 | 97 |
| EDTA plasma(n=5) | 91-99 | 95 |
| heparin plasma(n=5) | 99-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 87-101% | 78-98% | 81-105% |
| EDTA plasma(n=5) | 94-102% | 84-91% | 83-96% | 93-105% |
| heparin plasma(n=5) | 78-90% | 85-92% | 97-104% | 92-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。
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| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA551Ra01 | 堿性成纖維細胞生長因子(FGF2)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA551Ra01 | 堿性成纖維細胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA551Ra01 | 堿性成纖維細胞生長因子(FGF2)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA551Ra21 | 堿性成纖維細胞生長因子(FGF2)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA551Ra | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA551Ra | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA551Ra | 堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA551Ra11 | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UKSA551Ra01 | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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