Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Collagen Type II (COL2) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA572Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.2-200ng/mL
- 靈敏度最小可檢測劑量小于等于0.067 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-95 | 81 |
| EDTA plasma(n=5) | 92-105 | 102 |
| heparin plasma(n=5) | 98-105 | 101 |
| sodium citrate plasma(n=5) | 98-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-90% | 88-104% | 96-104% | 84-93% |
| EDTA plasma(n=5) | 80-97% | 98-105% | 79-92% | 96-103% |
| heparin plasma(n=5) | 85-104% | 85-94% | 86-99% | 98-105% |
| sodium citrate plasma(n=5) | 85-99% | 80-96% | 79-96% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UPAA572Ra01 | Ⅱ型膠原(COL2)多克隆抗體 | Testing in progress |
| ULAA572Ra71 | Ⅱ型膠原(COL2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA572Ra | Ⅱ型膠原(COL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA572Ra | Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis [PubMed: 22797069] |
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
| Spine (Phila Pa 1976). | No effects of Hyperosmolar Culture medium on Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells despite Upregulation Extracellular Matrix Genes. [Pubmed:25856264] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
| Materials Science and Engineering: C | Co-electrospun gelatin-poly (l-lactic acid) scaffolds: modulation of mechanical properties and chondrocyte response as a function of composition [Pubmed:24433895] |
| 義守大學生物技術與化學工程研究所學位論文 | 可拋棄式退化性關節(jié)炎電化學式尿液免疫感測器之研究 Electrochemical Immunosensor Detection of Urinary Biomaker for Osteoarthritis Diagnosis [Publication: U0074-2807201500521300] |
| Biomed Rep | Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs [PubMed: 26623021] |
| Macromolecular Bioscience | Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration [PubMed: 25787871] |
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310] |
| Cell Proliferation | Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes [Pubmed:27090063] |
| 中國眼耳鼻喉科雜志 | 白細胞介素10 上調表達對家兔慢性細菌性鼻竇炎黏膜創(chuàng)傷修復的影響 [article:11593] |
| Biomedical Materials | An?innovative?co-axial?system?to?electrospin?in?situ?crosslinked?gelatin?nanofibers. [Pubmed:26987305] |
| Scientific Reports | Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds [pubmed:27821864] |
| PLoS One. | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. [pubmed:28207788] |
| Polymers | Microsphere-Based Hierarchically Juxtapositioned Biphasic Scaffolds Prepared from Poly(Lactic-co-Glycolic Acid) and Nanohydroxyapatite for Osteochondral Tissue Engineering [2073-4360:8] |
| PLOS ONE | Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders [10.1371:journal.pone.0187564] |
| PLoS One | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders [10.1371:journal.pone.0172181] |
| Platelets. | Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate. [pubmed:29090639] |
| Molecular?Medicine?Reports | Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits [Pubmed:29257344] |
| Journal of Materials Science | Scaffolds modified with graphene as future implants for nasal cartilage [] |
| Processes | A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation [] |
