淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：?jiǎn)未位鞙y(cè)多因子不超過8個(gè)指標(biāo) ）
- 編號(hào)ULMA864Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)1.5h
- 檢測(cè)范圍0.98-1000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-102 | 95 |
| EDTA plasma(n=5) | 90-98 | 93 |
| heparin plasma(n=5) | 94-103 | 98 |
| sodium citrate plasma(n=5) | 88-95 | 91 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-99% | 93-105% | 78-98% | 78-90% |
| EDTA plasma(n=5) | 95-102% | 98-105% | 86-101% | 94-102% |
| heparin plasma(n=5) | 81-90% | 91-101% | 92-104% | 81-101% |
| sodium citrate plasma(n=5) | 78-104% | 78-93% | 88-99% | 79-101% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測(cè)溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測(cè)溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)和未標(biāo)記的淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競(jìng)爭(zhēng)結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA864Ra11 | 淀粉樣蛋白β1-40(Ab1-40)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA864Ra21 | 淀粉樣蛋白β1-40(Ab1-40)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA864Ra31 | 淀粉樣蛋白β1-40(Ab1-40)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA864Ra08 | 淀粉樣蛋白β1-40(Ab1-40)多克隆抗體 | WB; IHC; ICC; IP. |
| UCEA864Ra | 淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA864Ra | 淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA864Ra11 | 淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One | Metabolites of Cerebellar Neurons and Hippocampal Neurons Play Opposite Roles in Pathogenesis of Alzheimer's Disease [PubMed: PMC2677455] |
| Biochemical and Biophysical Research Communications | Antagonist of peroxisome proliferator-activated receptor γ induces cerebellar amyloid-β levels and motor dysfunction in APP/PS1 transgenic mice [ScienceDirect: S0006291X09008468] |
| Electromagnetic Biology and Medicine | Effect of 900 MHz Radio Frequency Radiation on Beta Amyloid Protein, Protein Carbonyl, and Malondialdehyde in the Brain [Informa: 15368378.2011.624654] |
| Electromagnetic Biology and Medicine | Do 100- and 500-μT ELF magnetic fields alter beta-amyloid protein, protein carbonyl and malondialdehyde in rat brains? [PubMed: 23324065] |
| Indian Journal of Experimental Biology | Intranasal administration of insulin lowers amyloid-levels in rat model of diabetes. [PubMed: 22279939] |
| European Journal of Pharmacology | The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1-42 seeding and short-term memory deficits in the Aβ25-35 mouse model of Alzheimer's disease. [ScienceDirect: S0014299912009028] |
| Neuropsychopharmacology | Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease [Pubmed: 23493042] |
| 世界科技研究與發(fā)展(World Sci-tech R & D) | 慢病毒介導(dǎo)的ＣＢＳ基因過表達(dá)對(duì)原代皮質(zhì)神經(jīng)元 [Globesci:Source] |
| Neurochemical research | Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain [Pubmed:24792732] |
| Neurochem Int | Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS1 transgenic model of Alzheimer's disease [PubMed: 25882165] |
| Journal of Alzheimer’s Parkinsonism & Dementia | The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats [open-access:] |
| Am J Alzheimers Dis Other Demen. | Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease [Pubmed:26993489] |
| Scientific Reports | Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s disease [articles:srep41041.ris] |
| Journal?of?Nutritional?Biochemistry | Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat diet [Pubmed:29986309] |
| Journal of Agricultural and?Food?Chemistry | Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic Mice [Pubmed:29695154] |
| Neurotoxicology | Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats [Pubmed:29522778] |
| Oncotarget | Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse?… [Pubmed:29731975] |
| Experimental?and?Therapeutic?Medicine | Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β?amyloid production and tau hyperphosphorylation [Doi: 10.3892/etm.2018.6860] |
| 非編碼RNA miR-137 調(diào)控tau 蛋白磷酸化和L 型鈣離子通道CACNA1C 參與阿爾茨海默病的發(fā)生 [] | |
| Neurotoxicity Research | Effect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 Cells [Pubmed: 30649678] |
| PLoS One | Obstructive sleep apnea may increase the risk of Alzheimer's disease [Pubmed: 31487291] |
| Frontiers in Aging Neuroscience | Prevention of Early Alzheimer's Disease by Erinacine A-Enriched Hericium erinaceus Mycelia Pilot Double-Blind Placebo-Controlled Study [Pubmed: 32581767] |
| PYRIMIDINE-4H-1OH DERIVATIVES RESTORE MITOCHONDRIAL FUNCTION IN EXPERIMENTAL CHRONIC TRAUMATIC ENCEPHALOPATHY [] | |
| Pharmacognosy Magazine | Mahanimbine-induced neuroprotection via cholinergic system and attenuated amyloidogenesis as well as neuroinflammation in lipopolysaccharides-induced?… [] |
| Neurochem Int | Lovastatin attenuates sevoflurane-induced cognitive disorder in aged rats via reducing A|? accumulation [34048842] |
| Brain Res Bul | Immunization with A|?3-10-KLH vaccine improves cognitive function and ameliorates mitochondrial dysfunction and reduces Alzheimer's disease-like pathology in Tg ?- [34044034] |
