胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Glucagon Like Peptide 1 (GLP1) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）
- 編號(hào)ULMA804Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)1.5h
- 檢測(cè)范圍0.9766-1000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.3255pg/mL.
- 樣本類(lèi)型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
- 價(jià)格 ¥ 1441 ¥ 1497 ¥ 1580 ¥ 1691 ¥ 1802 ¥ 1968 ¥ 2218 ¥ 2772 添加到價(jià)格計(jì)算器 計(jì)算器
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷(xiāo)商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-93 | 88 |
| EDTA plasma(n=5) | 81-88 | 84 |
| heparin plasma(n=5) | 86-96 | 92 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 92-105% | 82-96% | 92-101% |
| EDTA plasma(n=5) | 86-103% | 90-104% | 78-92% | 89-101% |
| heparin plasma(n=5) | 78-102% | 81-105% | 93-101% | 94-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測(cè)溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測(cè)溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)和未標(biāo)記的胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競(jìng)爭(zhēng)結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCEA804Ra | 胰高血糖素樣肽1(GLP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA804Ra | 抗胰高血糖素樣肽1抗體(Anti-GLP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| ULMA804Ra | 胰高血糖素樣肽1(GLP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA804Ra11 | 胰高血糖素樣肽1(GLP1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Diabetes, Obesity and Metabolism | Specificity and sensitivity of commercially available assays for glucagon‐like peptide‐1 (GLP‐1): implications for GLP‐1 measurements in clinical studies [Pubmed:25041349] |
| Acta Histochemica | Expression of cholecystokinin2-receptor in rat and human L cells and the stimulation of glucagon-like peptide-1 secretion by gastrin treatment [Pubmed:25601282] |
| Journal of Endocrinology | Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS [Pubmed:25630331] |
| Obes Surg | Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model [PubMed: 26202420] |
| International Journal of Pharmacognosy and Phytochemical Research | Anti-hiperglycemic Effect of Urena lobata Leaf Extract by Inhibition of Dipeptidyl Peptidase IV (DPP-IV) on Diabetic Rats [Pdf: 7] |
| European Journal of Pharmacology | Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model [Pubmed:27131827] |
| Int J Obes | miR-200a reCavia (Guinea pig )lates Rheb-mediated amelioration of insulin resistance after duodenal-jejunal bypass [Pubmed:27121251] |
| Journal of Traditional and Complementary Medicine | Incretin effect of Urena lobata leaves extract on structure and function of rats islet β-cells [S2225411016302802] |
| World?Journal of Gastroenterology? | Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats [pubmed:27547013] |
| World?Journal of Gastroenterology | Effects?of?sleeve?gastrectomy?with?jejuno-jejunal?or?jejuno-ileal?loop?onglycolipid?metabolism?in?diabetic?rats. [pubmed:27621579] |
| European?Journal?of?Nutrition | Improvement?in?glucose?tolerance?and?insulin?sensitivity?by?probiotic?strains?of?Indian?gut?originin?high-fat?diet-fed?C57BL/6J?mice. [pubmed:27757592] |
| Journal of Traditional and Complementary Medicine? | Incretin effect of Urena lobata leaves extract on structure and function of rats islet b-cells [d69b5594c032d557c3f3203b91aba2604a94] |
| Metabolism | Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2 diabetes [10.1016/j.metabol.2017.10.015] |
| Obesity surgery | The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats. [pubmed:28840471] |
| Obesity?Surgery | Selective Vagotomy Worsens Glucose Control After Ileal Transposition [Pubmed:29525935] |
| Obesity?Surgery? | Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague?… [Pubmed:29098544] |
| Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
| Journal of Physiology and Biochemistry | Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model [Pubmed:29781038] |
| Metabolism | Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2?… [Pubmed:29129820] |
| Nutrition in the Prevention and Treatment of Abdominal Obesity | Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig [] |
| Peptides | The influence of high fat diet on plasma incretins and insulin concentrations in Sprague-Dawley rats with diet-induced obesity and glucose intolerance undergoing?… [Doi: 10.1016/j.peptides.2019.04.001] |
| The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811] |
| Neuropsychobiology | Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol Dependence [Pubmed: 31117084] |
| OBESITY SURGERY | The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats [Pubmed: 31701411] |
| Journal of Lipid Research | Novel GPR120 agonist TUG891 modulates fat taste perception and preference and activates tongue-brain-gut axis in mice [Pubmed: 31806728] |
| Metabolik sendromlu s??anlarda yüzme egzersizinin plazma glukagon-benzeri PEPT?D-1 (GLP-1) seviyesi ve hipokampal GLP-1 resept?rü üzerine etkisinin?… [] | |
| World J Diabetes | Effect of oligofructose on resistance to postoperative high-fat diet-induced damage of metabolism in diabetic rats after sleeve gastrectomy [33889290] |
| Study on the Therapeutic Effects of Traditional Chinese Medicine on Insulin Resistance in Obese Mice by Modulating Intestinal Function [] | |
| Am J Physiol Endocrinol Metab | Central GLP-1 contributes to improved cognitive function and brain glucose uptake after duodenum-jejunum bypass on obese and diabetic rats [34370593] |
| Surgical Endoscopy | The effect of bariatric surgery on the expression of Growth Differentiation Factor-15/Macrophage-Inhibitory Cytokine-1 (GDF-15/MIC-1) in rat [Pubmed:35029766] |
| Sci Rep | The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairment [Pubmed:35468904] |
| Nutr Diabetes | Response of blood glucose and GLP-1 to different food temperature in normal subject and patients with type 2 diabetes [Pubmed:35624116] |
| Different Estradiol Level's Impact on Appetite Regulation, Food Intake, and Concentrations of Leptin, Glucagon-Like Peptide-1, and High-Sensitivity C-Reactive?… [] |
