結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)
High Sensitive ELISA Kit for Haptoglobin (Hpt)
HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin
- 編號UHEA817Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.102ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1040 ¥ 1485 ¥ 6683 ¥ 12623 ¥ 103950
- 欲了解實(shí)際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-97 | 93 |
| EDTA plasma(n=5) | 78-92 | 80 |
| heparin plasma(n=5) | 98-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-103% | 83-90% | 97-104% | 92-99% |
| EDTA plasma(n=5) | 87-95% | 85-99% | 88-99% | 95-103% |
| heparin plasma(n=5) | 86-95% | 95-102% | 91-99% | 90-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗員來進(jìn)行操作可減少人為誤差。
實(shí)驗流程
1. 實(shí)驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗原理
將結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA817Hu01 | 結(jié)合珠蛋白(Hpt)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA817Hu03 | 結(jié)合珠蛋白(Hpt)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA817Hu04 | 結(jié)合珠蛋白(Hpt)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA817Hu02 | 結(jié)合珠蛋白(Hpt)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA817Hu01 | 結(jié)合珠蛋白(Hpt)多克隆抗體 | WB; IHC |
| UPAA817Hu03 | 結(jié)合珠蛋白(Hpt)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA817Hu02 | 結(jié)合珠蛋白(Hpt)多克隆抗體 | WB; IHC |
| UPAA817Hu04 | 結(jié)合珠蛋白(Hpt)多克隆抗體 | WB |
| ULAA817Hu71 | 結(jié)合珠蛋白(Hpt)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA817Hu29 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | IHC |
| UMAA817Hu22 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB; IHC |
| UMAA817Hu21 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB; IHC |
| UMAA817Hu23 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB; IHC |
| UMAA817Hu24 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB; IHC |
| UMAA817Hu25 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB; IHC |
| UMAA817Hu27 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB; IHC |
| UMAA817Hu28 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB |
| UMAA817Hu26 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB |
| UFAA817Hu06 | 抗結(jié)合珠蛋白(Hpt)單克隆抗體 | FCM |
| UFAA817Hu02 | 抗結(jié)合珠蛋白(Hpt)單克隆抗體 | FCM |
| UFAA817Hu04 | 抗結(jié)合珠蛋白(Hpt)單克隆抗體 | FCM |
| ULAA817Hu72 | 結(jié)合珠蛋白(Hpt)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA817Hu | 結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA817Hu | 結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA817Hu | 結(jié)合珠蛋白(Hpt)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA817Hu | 結(jié)合珠蛋白(Hpt)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA817Hu01 | 結(jié)合珠蛋白(Hpt)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA817Hu01 | 結(jié)合珠蛋白(Hpt)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Vascular Surgery | Circulation levels of acute phase proteins in patients with Takayasu arteritis [PubMed: 20100644] |
| Genetics | Quantitative trait loci mapping of the mouse plasma proteome (pQTL). [NCBI: PMC3567747] |
| ostaglandins Leukot Essent Fatty Acids. | Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. [Pubmed: 24095588] |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| Annals of Neurology? | High complement levels in astrocyte‐derived exosomes of Alzheimer disease [Pubmed:29406582] |
| European Journal of Medicinal Plants | Effects of Hippophae rhamnoides L. Leaf and Marc Extract with Reduced Tannin Concentration on the Health and Growth Parameters of Newborn Calves [] |
| Journal?of?Animal?Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood?… [Pubmed: 30184125] |
| Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327] |
| International Journal of Biological Sciences | Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163 [] |
| Food and Chemical Toxicology | Prolyl endopeptidase-degraded low immunoreactive wheat flour attenuates immune responses in Caco-2 intestinal cells and gluten-sensitized BALB/c mice [Pubmed: 31082461] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| International Journal of Biological Sciences | Deletion of CD163 Exon 7 Confers Resistance to Highly Pathogenic Porcine Reproductive and Respiratory Viruses on Pigs [] |
| Pediatric Obesity | Intestinal permeability and small intestine bacterial overgrowth in excess weight adolescents [Pubmed: 33089672] |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient??- [33474564] |
| large animal review | Influence of niacin application on inflammatory parameters, non-esterified fatty acids and functional status of liver in cows during early lactation [] |
| PLoS One | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? [34170951] |
| Probiotics Antimicrob Proteins | The Effect of Feed Supplementation with EM Bokashi? Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators?… [34596883] |
