硒蛋白P1(SEPP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Selenoprotein P1, Plasma (SEPP1)
SEPP; SeP; SEP-P1; SELP
- 編號(hào)USEB809Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測(cè)范圍31.2-2,000ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于12.1ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1516 ¥ 2166 ¥ 9747 ¥ 18411 ¥ 151620
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)硒蛋白P1(SEPP1)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的硒蛋白P1(SEPP1)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-95 | 90 |
| EDTA plasma(n=5) | 96-105 | 102 |
| heparin plasma(n=5) | 83-90 | 86 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的硒蛋白P1(SEPP1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中硒蛋白P1(SEPP1)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-96% | 89-96% | 81-105% | 98-105% |
| EDTA plasma(n=5) | 86-101% | 80-96% | 93-105% | 96-104% |
| heparin plasma(n=5) | 87-94% | 91-101% | 80-99% | 90-101% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將硒蛋白P1(SEPP1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的硒蛋白P1(SEPP1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的硒蛋白P1(SEPP1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的硒蛋白P1(SEPP1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB809Ra01 | 硒蛋白P1(SEPP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB809Ra01 | 硒蛋白P1(SEPP1)多克隆抗體 | WB |
| UMAB809Ra21 | 硒蛋白P1(SEPP1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEB809Ra | 硒蛋白P1(SEPP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB809Ra | 硒蛋白P1(SEPP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Journal of Clinical Endocrinology & Metabolism | Serum Selenoprotein P Levels in Patients with Type 2 Diabetes and Prediabetes: Implications for Insulin Resistance, Inflammation, and Atherosclerosis [PubMed: 21677040] |
| Diabetes & metabolism journal | Increased Selenoprotein P Levels in Subjects with Visceral Obesity and Nonalcoholic Fatty Liver Disease [PubMed: 23439771] |
| Journal of Pediatric Biochemistry | Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemia [Metapress:Source] |
| Journal of Trace Elements in Medicine and Biology | Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism [PubMed: 26854239] |
| OBES RES CLIN PRACT/OBESITY RESEARCH & CLINICAL PRACTICE | Selenoprotein?P?is?elevated?in?individuals?with?obesity, but is not?independently?associated?withinsulin?resistance. [pubmed:27524654] |
| Annals of Nutrition and Metabolism | Acute?Overfeeding?Does Not Alter Liver or?Adipose?Tissue-Derived?Cytokines?in?HealthyHumans. [pubmed:27832637] |
| European?Journal?of?Nutrition | DNA?damage?and?oxidative?stress?response?to?selenium?yeast?in the?non-smoking?individuals: ashort-term?supplementation?trial?with?respect?to?GPX1?and SEPP1 polymorphism. [pubmed:26658762] |
| South. Clin. Ist. Euras. | Evaluation of the Relationship Between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary Syndrome [DOI: 10.14744/scie.2017.87597] |
| Arch Endocrinol Metab | Serum selenium and selenoprotein-P levels in autoimmune thyroid dieases patients in a select center a transversal study [DOI: 10.1590/2359-3997000000309] |
| British Journal of Nutrition | Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth [pubmed:28534447] |
| Journal of?Trace?Elements?in Medicine and Biology | Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury [Pubmed:29895371] |
| Biological &?Pharmceutical Bulletin | Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits [Pubmed:29709922] |
| Diabetologia | Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitus [Pubmed: 30470912] |
| Biological Trace Element Research | Selenium Levels in Community Dwellers with Type 2 Diabetes Mellitus [Pubmed: 30725267] |
| Advances in?Medical?Sciences | Can hepatokines be regarded as novel non-invasive serum biomarkers of intrahepatic lipid content in obese children? [Pubmed: 30921653] |
| Acute swimming exercise, but not exposure to moderate hypoxic conditions reduces circulating selenoprotein P levels in short-term, high-fat diet-fed rats [Pubmed: 31236416] | |
| Hormones | Selenium and selenoprotein P in nonalcoholic fatty liver disease [Pubmed: 31493247] |
| Bull Environ Contam Toxicol | Soil Selenium Concentration and Residents Daily Dietary Intake in a Selenosis Area: A Preliminary Study in Yutangba Village, Enshi City, China [Pubmed: 32909074] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Higher Serum Selenoprotein P Level as a Novel Inductor of Metabolic Complications in Psoriasis [Pubmed: 32605214] |
| Circulating hepassocin level in patients with stable angina is associated with fatty liver and renal function [] | |
| Nat Immunol | Selenium–GPX4 axis protects follicular helper T cells from ferroptosis [34413521] |
| Reviews in Cardiovascular Medicine | Selenoprotein P-1 (SEPP1) as An Early Biomarker of Acute Kidney Injury in Patients Undergoing Cardiopulmonary Bypass [] |
