腎酶(RNLS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Renalase (RNLS)
MAO-C; C10orf59; FAD-Dependent Amine Oxidase; Monoamine oxidase-C; alpha-NAD(P)H oxidase/anomerase
- 編號USEC845Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍1.56-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.65ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1596 ¥ 2280 ¥ 10260 ¥ 19380 ¥ 159600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測腎酶(RNLS)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的腎酶(RNLS)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-104 | 94 |
| EDTA plasma(n=5) | 89-103 | 93 |
| heparin plasma(n=5) | 85-101 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的腎酶(RNLS)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腎酶(RNLS)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-94% | 80-97% | 79-98% | 97-105% |
| EDTA plasma(n=5) | 88-99% | 94-101% | 84-102% | 86-95% |
| heparin plasma(n=5) | 84-98% | 93-103% | 90-97% | 78-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將腎酶(RNLS)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的腎酶(RNLS)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的腎酶(RNLS)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的腎酶(RNLS)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC845Ra01 | 腎酶(RNLS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC845Ra01 | 腎酶(RNLS)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAC845Ra21 | 腎酶(RNLS)單克隆抗體 | WB; IHC; ICC; IP. |
| USEC845Ra | 腎酶(RNLS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC845Ra | 腎酶(RNLS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Pol Arch Med Wewn. | Renalase, kidney function, and markers of endothelial dysfunction in renal transplant recipients [Cartelcolor: source] |
| Angiology | Renalase, Stroke, and Hypertension in Hemodialyzed Patients [PubMed: 22583169] |
| Peritoneal dialysis international | Renalase in peritoneal dialysis patients is not related to blood pressure, but to dialysis vintage. [Pdiconnect: Source] |
| Archives of Medical Science | Vascular adhesion protein-1 and renalase in regard to diabetes in hemodialysis patients [PubMed: PMC3542495] |
| Renal Failure | Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients [Pubmed: 23530612] |
| Borgis - Postepy Nauk Medycznych | VAP-1 in chronic kidney disease and diabetic kidney disease [Czytelniamedyczna: Source] |
| Journal of Basic and Clinical Physiology and Pharmacology | The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy [Pubmed: 15365253] |
| Exp Biol Med (Maywood). | Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients. [Pubmed: 24599883] |
| Kidney Blood Press Res. | Renalase, a novel enzyme involved in blood pressure regulation, is related to kidney function but not to blood pressure in hemodialysis patients. [Pubmed: 22539018] |
| Nephrology Dialysis Transplantation | Renalase in hypertension and kidney disease [Oxfordjournals: Source] |
| Lipids in Health and Disease | Markers of increased cardiovascular risk in patients with chronic kidney disease [Biomedcentral:Source] |
| Research & Experimental? | Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients [Pubmed:24599883] |
| Pediatr Nephrol | Normal values for urine renalase excretion in children [Pubmed:25060760] |
| J Am Soc Hypertens. | Low plasma renalase concentration in hypertensive patients after surgical repair of coarctation of aorta [Pubmed:25064768] |
| Cardiology. | Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults [Pubmed:25824645] |
| J Am Soc Hypertens | Circulating renalase, catecholamines, and vascular adhesion [PubMed: 26403854] |
| Indian Pediatr | Renalase levels in children with solitary functioning kidney [PubMed: 26713989] |
| Oxford Journals | SP321 RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS [Content: 30] |
| journal of hypertension | PP.27.07: PREHYPERTENSIVE ALTERATIONS OF ARTERIAL AND VENOUS REACTIVITY IN A RAT POLYCYSTIC OVARY SYNDROME MODEL. EFFECTS OF PARALLEL VITAMIN D3 ADMINISTRATION [Jhypertension: Abstract] |
| journal of hypertension | PP.27.06: RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS. [Jhypertension: Abstract] |
| journal of hypertension | PP.27.04: ENDOTHELIAL DYSFUNCTION AND ARTERIAL HYPERTENSION IN THE WORKING POPULATION (MERIDIAN STUDY). [Jhypertension: Abstract] |
| Curr Vasc Pharmacol. | Renalase is Removed by Kidneys and During Dialysis – Excess Related to CKD Complications? [PubMed: 25515790] |
| Kidney Blood Press Res | Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt Diet [PubMed: 26619289] |
| PLoS One | Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis [PubMed: 26431044] |
| J Hum Hypertens | Plasma renalase concentration before and after radiofrequency renal denervation in patients with resistant hypertension [PubMed: 26310184] |
| Nephrology (Carlton) | Urinary renalase concentration in patients with preserved kidney function undergoing coronary angiography. [pubmed:27778420] |
| Nephrol Dial Transplant | PLASMA RENALASE CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION: PRELIMINARY RESULTS [content:31] |
| Renal?Failure | Increased?serum?renalase?in?hemodialysis?patients: is it?related?to?left ventricular hypertrophy? [pubmed:27416751] |
| Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implants | High-performance Liquid Chromatography?Measured?Metabolites?of?EndogenousCatecholamines and Their Relations to Chronic Kidney Disease and?High?Blood?Pressure?in Heart Transplant Recipients. [pubmed:27496485] |
| PLOS?ONE | Online Haemodiafiltration Improves Inflammatory State in Dialysis Patients: A Longitudinal Study. [pubmed:27783636] |
| Advances in Experimental Medicine and Biology | Renalase in Children with Glomerular Kidney Diseases [chapter:10.1007] |
| Kidney & Blood Pressure Research | Plasma Renalase is Not Associated with Blood Pressure and Brachial-Ankle Pulse Wave Velocity in Chinese Adults With Normal Renal Function [452587] |
| BMC nephrology | Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls [pubmed:28372594] |
| Nefrología (English Edition) | Increased serum renalase in peritoneal dialysis patients: Is it related to cardiovascular disease risk [pubmed:28160962] |
| Clinical?Interventions?in?Aging | Age influence on renalase and catecholamines concentration in hypertensive patients, including maintained dialysis [pubmed:27822026] |
| International Journal of?Clinical?and Experimental?Medicine | Aumento de renalasa sérica en pacientes de diálisis peritoneal:? está relacionado con el riesgo de enfermedad cardiovascular? [10.1016:j.nefro.2016.11.013] |
| PLoS One | Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients [pubmed:28614373] |
| Annals of Agricultural and Environmental Medicine | Levels of renalase and advanced oxidation protein products with regard to catecholamines in haemodialysed patients [pubmed:28954489] |
| Angiology | Endocan Concentration in Patients With Primary Hypertension [pubmed:29084442] |
| Journal of Hypertension | RENALASE PLASMA CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION [10.1097/01.hjh.0000523893.07419.ae] |
| International journal of molecular sciences | Renalase Protects against Renal Fibrosis by Inhibiting the Activation of the ERK Signaling Pathways [pubmed:28448446] |
| Journal of sports medicine and physical fitness | Transient changes in serum renalase concentration during long-distance running: The case of an amateur runner under continuous training [10.7600/jpfsm.6.159] |
| Scientific Reports | Synergistic effect of renalase and chronic kidney disease on endothelin-1 in patients with coronary artery disease?a cross-sectional study [Pubmed:29743680] |
| Life?Sciences | Influence of acute exercise on renalase and its regulatory mechanism [Pubmed:30056018] |
| Experimental?Eye?Research | Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration [Pubmed:29580721] |
| Rapid?Communications?in?Mass?Spectrometry | A new method for quantitative determination of renalase based on mass‐spectrometry determination of a proteotypic peptide labeled with stable isotopes [10.1002:rcm.8167] |
| International?Urology?and?Nephrology | Renalase contributes to protection against renal fibrosis via inhibiting oxidative stress in rats [Pubmed:29476431] |
| Pediatric Cardiology | Serum Renalase Levels in Adolescents with Primary Hypertension [Pubmed:29748702] |
| Postepy?w Kardiologii?Interwencyjnej | Atorvastatin facilitates protection against contrast-induced nephropathy in patients undergoing coronary angiography via humoral mediators rather than?… [Pubmed:30008773] |
| High urinary kidney injury molecule type 1 level is associated with 12-month adverse cardiovascular events in patients with coronary artery disease [publication:324522368] | |
| Journal of?Clinical?Medicine | Serum Renalase Levels Are Predicted by Brain-Derived Neurotrophic Factor and Associated with Cardiovascular Events and Mortality after Percutaneous Coronary?… [Pubmed: 30424498] |
| Archives of disease in childhood | GP23 Percutaneous closure of patent ductus arteriosus: single center experience [] |
| Archives of disease in childhood | GP25 Comparison of associations and outcomes of prenatally and postnatally diagnosed right aortic arch: south wales' experience [] |
| Archives of disease in childhood | GP24 Serum renalase levels in adolescents with primary hypertension [] |
| Disease Markers | Association of Plasma Renalase and Left Ventricle Mass Index in Heart Failure Patients Stratified to the Category of the Ejection Fraction: A Pilot Study [Pubmed: 31737132] |
| EXPERIMENTAL BIOLOGY AND MEDICINE | The role of urinary renalase on early-stage renal damage in Chinese adults with primary hypertension [Pubmed: 32102561] |
| FEBS Open Bio | Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats [Pubmed: 32053739] |
| current medical research and opinion | The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patients [Pubmed: 32297799] |
| Dovepress | Association of Serum Renalase Levels and Renalase rs10887800 Polymorphism with Unstable Angina Pectoris Patients Having Metabolic Syndrome [Pubmed: 32982355] |
| 學(xué)位論文 | Nikotinamidadenindinukleotid-Blutplasmawerte sind unabh?ngig vom kalendarischen und vaskul?ren Alter in einer Normalkohorte aus der Region Halle-Saalekreis [] |
| Life | Circulating Renalase as Predictor of Renal and Cardiovascular Outcomes in Pre-Dialysis CKD Patients: A 5-Year Prospective Cohort Study [33800219] |
| Edited by: Background: Renalase has been implicated in chronic heart failure (CHF); however, nothing is known about renalase discriminatory ability and prognostic?… [] |
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