免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Immunoglobulin G (IgG)
- 編號UCEA544Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長2h
- 檢測范圍1.23-100ug/mL
- 靈敏度最小可檢測劑量小于等于0.49ug/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 945 ¥ 1350 ¥ 6075 ¥ 11475 ¥ 94500
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測免疫球蛋白G(IgG)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的免疫球蛋白G(IgG)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 87-98 | 94 |
| heparin plasma(n=5) | 80-93 | 81 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的免疫球蛋白G(IgG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白G(IgG)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-92% | 96-105% | 95-105% | 79-90% |
| EDTA plasma(n=5) | 94-105% | 94-101% | 89-97% | 97-105% |
| heparin plasma(n=5) | 83-95% | 87-103% | 93-102% | 80-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將免疫球蛋白G(IgG)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPA544Ra01 | 免疫球蛋白G(IgG)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA544Ra01 | 免疫球蛋白G(IgG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| USAA544Ra00 | 兔抗大鼠IgG多克隆抗體(無標(biāo)記) | WB |
| UPAA544Ra01 | 免疫球蛋白G(IgG)多克隆抗體 | WB; IHC |
| UPAA544Ra02 | 免疫球蛋白G(IgG)多克隆抗體 | IHC |
| USAA544Ra06 | 兔抗大鼠IgG多克隆抗體(吲哚菁綠標(biāo)記) | WB; IHC; ICC; IF. |
| USAA544Ra05 | 兔抗大鼠IgG多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | WB; IHC; ICC; IF. |
| USAA544Ra04 | 兔抗大鼠IgG多克隆抗體(藻紅素標(biāo)記) | WB; IHC; ICC; IF. |
| USAA544Ra09 | 兔抗大鼠IgG多克隆抗體(辣根過氧化物酶標(biāo)記) | WB |
| USAA544Ra07 | 兔抗大鼠IgG多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USAA544Ra08 | 兔抗大鼠IgG多克隆抗體(異硫氰酸熒光素標(biāo)記) | ICC/IF |
| UMAA544Ra22 | 免疫球蛋白G(IgG)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA544Ra21 | 免疫球蛋白G(IgG)單克隆抗體 | WB |
| UHEA544Ra | 免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA544Ra | 免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA544Ra | 免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA544Ra | 免疫球蛋白G(IgG)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA544Ra | 免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA544Ra11 | 免疫球蛋白G(IgG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Animal and Veterinary Advances | Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616] |
| Journal of Animal and Veterinary Advances | Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600] |
| international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
| Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ] |
| Pharmaceutical Development and Technology | Foot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristics [Pubmed: 23590209] |
| BioMed Research International | The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling [Hindawi:Source] |
| Journal of Proteomics | Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection [Pubmed:24576640] |
| Int J Occup Environ Health. | Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors [Pubmed:24999850] |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407] |
| Oncology Reports | Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer [Pubmed:25529861] |
| Biomed Res Int | The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling [PubMed: 26161394] |
| Protein Pept Lett | Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend Microspheres [PubMed: 26268563] |
| Electrophoresis | Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27 [Pubmed:26914286] |
| Neuropeptides | Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro. [Pubmed:26707636] |
| Archives of Biological Sciences | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine [pid:46641600021Z] |
| Asian J Pharm Clin Res | INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790] |
| journal of biotechnology | Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans. [pubmed:28013072] |
| Sci Rep.? | Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. [pubmed:28220870] |
| Toxicology Research | Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis [C6TX00288A] |
| ntervirology | Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide [pubmed:27694750] |
| Biomedicine?&?Pharmacotherapy | Icariin?promotes?osteogenic?differentiation?of?rat?bone?marrow?stromal?cells?by?activating?the ERα-Wnt/β-catenin?signaling?pathway. [pubmed:27764755] |
| Food and Agricultural Immunology | Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers [10.108:09540105.2017.1311308 ] |
| Biomedicine & Pharmacotherapy | In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats [pubmed:29091887] |
| Journal of Biochemical and Molecular Toxicology | Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein [10.1002/jbt.21912] |
| International Journal of Science and Research | Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus Bacteria [ISSN: 2319-7064] |
| Molecular Medicine Reports | Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. [pubmed:28586019] |
| Revista Brasileira de Zootecnia | Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders [10.1590/s1806-92902017000200004?] |
| Water?Research? | Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095] |
| Clinical?Immunology | Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis [Pubmed:30056130] |
| Food and Agricultural Immunology | Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers [10.1080:09540105.2018.1457013] |
| Journal of Molecular Neuroscience | A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related Enzymes [Pubmed:30062438] |
| Biological?Trace?Element?Research | Supplementation of Cr Methionine During Dry Period of Dairy Cows and Its Effect on Some Production and Biochemical Parameters During Early Lactation and on?… [Pubmed:29497999] |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a?… [Pubmed:29305686] |
| SAGE?Open?Medicine | Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes [Pubmed:29623201] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167] |
| ACS?Applied?Materials?&?Interfaces | Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications [Pubmed: 30433758] |
| Molecular?Human?Reproduction | Phenotypic variations in transferred progeny due to genotype of surrogate mother: Surrogate mother modulates transferred progeny [Pubmed: 30445548] |
| Bioscience, Biotechnology, and Biochemistry | In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6 [] |
| Journal?of?proteome?research | Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection [] |
| International Immunopharmacology | Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice [Pubmed: 30974284] |
| Utjecaj dodatka selena u prihrani na hematolo?ku sliku i biokemijske pokazatelje u jelena lopatara (Dama dama L.) [] | |
| Biogerontology | Age-related chemokine alterations affect IgA secretion and gut immunity in female mice [Pubmed: 32277312] |
| International Journal of Biological Macromolecules | Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens [Pubmed: 32224178] |
| Pharmaceutics | Efficacy of Local Minocycline Agents in Treating Peri-Implantitis: An Experimental In Vivo Study in Beagle Dogs [Pubmed: 33114168] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharide [Pubmed: 33010421] |
| Pharmaceutics | Locally Applied Slow-Release of Minocycline Microspheres in the Treatment of Peri-Implant Mucositis: An Experimental In Vivo Study [Pubmed: 32708741] |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
| Molecular Medicine Reports | Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3?E1 cells via an autocrine mechanism [Pubmed: 33000198] |
| South African Journal of Botany | Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo [] |
| Journal of Functional Foods | Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts [] |
| Italian Journal of Animal Science | Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the?… [] |
| Regenerative Biomaterials | A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model [] |
| ACS APPLIED BIO MATERIALS | Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy [] |
| 博士論文 | Women Scientist Scientist Mentor [] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |
| THERANOSTICS | Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without??- [33456566] |
| Life Sci | Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats [33577848] |
| Animals (Basel) | Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets. Animals 2021, 11, 404 [33562568] |
| Environ Pollut | Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073] |
| Veterinary Microbiology | Optimized GAPDH-truncated immunogen of Streptococcus equi elicits an enhanced immune response and provides effective protection in a mouse model [33647714] |
| Experimental and Therapeutic Medicine | Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats [33574912] |
| British Journal of Pharmacology | The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus [] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle [] |
| Neonatal Buza??larda ?ki Farkl? ?mmunomod¨1lat?r¨1n Buza?? ?shalleri ¨1zerine Etkileri [] | |
| Microbiology Spectrum | Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows [34494854] |
| Pediatrics International | Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus [34559910] |
| Toxicology | Protection from Benzene-induced Immune Dysfunction in Mice [Pubmed:35090963] |
| Food Funct | Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093] |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death [Pubmed:35732625] |
| Nature Communications | Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction [Pubmed:35668082] |
| Food and Agricultural Immunology | The effect of the multi-strain probiotic preparation EM Bokashi? on selected parameters of the cellular immune response in pigs [] |
| 陸軍軍醫(yī)大學(xué)學(xué)報(bào) | 信筒子醌對系統(tǒng)性紅斑狼瘡小鼠免疫調(diào)節(jié)和治療作用 [] |
留言咨詢
