腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Brain Natriuretic Peptide (BNP)
GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32
- 編號(hào)UCEA541Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)2h
- 檢測(cè)范圍4.94-400pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于1.82pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1407 ¥ 2010 ¥ 9045 ¥ 17085 ¥ 140700
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)腦鈉素(BNP)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的腦鈉素(BNP)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-103 | 98 |
| EDTA plasma(n=5) | 79-101 | 89 |
| heparin plasma(n=5) | 79-94 | 88 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的腦鈉素(BNP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中腦鈉素(BNP)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-99% | 96-105% | 88-96% | 87-101% |
| EDTA plasma(n=5) | 82-95% | 91-104% | 90-99% | 83-90% |
| heparin plasma(n=5) | 91-105% | 89-97% | 78-103% | 94-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測(cè)液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將腦鈉素(BNP)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原(標(biāo)準(zhǔn)品或樣本)，待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過(guò)溫育和徹底洗滌后加入底物TMB顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測(cè)標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測(cè)物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（O.D.值），計(jì)算樣品濃度。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA541Ra01 | 腦鈉素(BNP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA541Ra01 | 腦鈉素(BNP)多克隆抗體 | IHC |
| UMAA541Ra21 | 腦鈉素(BNP)單克隆抗體 | IHC |
| UMAA541Ra22 | 腦鈉素(BNP)單克隆抗體 | IHC |
| UMEA541Ra | 腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA541Ra | 腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UWEA541Ra | 腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA541Ra | 腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA541Ra | 腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA541Ra11 | 腦鈉素(BNP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Heart and Circulatory Physiology | B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source] |
| PLoS One | Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation. [PubMed: 21297953] |
| International Journal of Toxicology | Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in Rats [Pubmed: 24055824] |
| JACC | ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHY [Onlinejacc:Source] |
| J Urol.? | Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing? [Pubmed:24679871] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca2 [PubMed: 26706290] |
| Vlaams Diergeneeskundig Tijdschrift | Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study [Publication: 5950761] |
| Physiological Research | Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro [Pubmed:26706290] |
| J Ethnopharmacol | Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway [Pubmed:26970569] |
| Echocardiography. | Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction. [pubmed:27779336] |
| SpringerPlus | The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … [pubmed:27652141] |
| Heart Lung Circ. | Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054] |
| Clinica?Chimica?Acta? | Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy [pubmed:27693530] |
| Scientific Reports | Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP. [pubmed:28091624] |
| Molecular Medicine Report | Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failure [pubmed:28627670] |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788] |
| Acta Physiologica | Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload [pubmed:27575955] |
| International Immunopharmacology | Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK [pubmed:28410530] |
| Drug Delivery | Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy. [pubmed:29063791] |
| Science?of the?Total?Environment | Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991] |
| Scientific Reports | An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease [Pubmed:29895976] |
| International Journal of Cardiology | Cardioprotective effect of substance P in a porcine model of acute myocardial infarction [Pubmed:29884290] |
| International?Journal?of?Cardiology | High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failure [Pubmed:30072155] |
| Evidence-based?Complementary and Alternative?Medicine? | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313] |
| Molecular?Medicine?Reports? | Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781] |
| Pediatric Cardiology | Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease [Pubmed:29392348] |
| BMC?Pharmacology and Toxicology | Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis [Pubmed:29321061] |
| 中國(guó)病理生理雜志 | 和肽素水平對(duì)大鼠心腎綜合征的早期預(yù)測(cè)價(jià)值 [filename:ZBLS201802013] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| Toxicology Mechanisms and?Methods | Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860] |
| Van Veterinary Journal | Van Veterinary Journal [] |
| Kidney?International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217] |
| Journal?of?Cellular?Physiology | Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes [Pubmed: 30982981] |
| Cardiology | Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure [Pubmed: 31466059] |
| BIOMEDICINE & PHARMACOTHERAPY | Pharmacodynamic and pharmacokinetic effects of S086, a novel angiotensin receptor neprilysin inhibitor [Pubmed: 32570118] |
| biochimica et biophysica acta-molecular basis of disease | Empagliflozin improves left ventricular diastolic function of db/db mice [Pubmed: 32353614] |
| Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF|?1/Smad ?- [34116674] | |
| Radiation-induced heart energy metabolism dysfunction leads to heart fibrosis [] | |
| Oxid Med Cell Longev | κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a [34373768] |
| Mol Med Rep | Radiation?induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues [34633055] |
| viruses | Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model [34452454] |
| Peptides | Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress [Pubmed:35218795] |
