前列腺素E2(PGE2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Prostaglandin E2 (PGE2)
PG-E2
- 編號UCEA538Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍24.69-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于8.67pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1671 ¥ 2387 ¥ 10741 ¥ 20288 ¥ 167076
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測前列腺素E2(PGE2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的前列腺素E2(PGE2)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 99-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的前列腺素E2(PGE2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中前列腺素E2(PGE2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-102% | 78-104% | 93-105% | 78-96% |
| EDTA plasma(n=5) | 78-101% | 91-98% | 95-103% | 79-103% |
| heparin plasma(n=5) | 91-101% | 81-96% | 79-96% | 88-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將PGE2抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原（標(biāo)準(zhǔn)品或樣本），待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA538Ge11 | 前列腺素E2(PGE2)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA538Ge01 | 前列腺素E2(PGE2)多克隆抗體 | ELISA |
| UMAA538Ge27 | 前列腺素E2(PGE2)單克隆抗體 | ELISA |
| UMAA538Ge21 | 前列腺素E2(PGE2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA538Ge22 | 前列腺素E2(PGE2)單克隆抗體 | ELISA |
| UMEA538Ge | 前列腺素E2(PGE2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA538Ge | 前列腺素E2(PGE2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA538Ge | 前列腺素E2(PGE2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Immunopharmacology and Inflammation | Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid [ScienceDirect: S0188440910001049] |
| PLoS ONE | Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis [PubMed: PMC3724827] |
| Lipids in Health and Disease | Lipid mediators in plasma of autism spectrum disorders [PubMed: PMC3557222] |
| Journal of Thoracic Oncology | Interleulin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage-dominant Microenvironment in Lung Cancer [PubMed: PMC3378786] |
| Cell biology international, | Artesunate exerts an anti-immunosuppressive effect on cervical cancer by inhibiting PGE2 production and Foxp3 expression. [Pubmed: 24446394] |
| Journal of ethnopharmacology | Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. [Pubmed: 24211397] |
| Journal of Gastroenterology and Hepatology | Celecoxib attenuates hepatic cirrhosis through inhibition of epithelial-to-mesenchymal transition of hepatocytes [Pubmed:24909904] |
| J Surg Res. | Valproic acid protects septic mice from renal injury by reducing the inflammatory response [Pubmed:24948542] |
| Inflammation.? | Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway. [Pubmed:24962643] |
| Mol Med Rep | Somatostatin prevents lipopolysaccharide [PubMed: 25777539] |
| Eur J Pharmacol | Celecoxib enhanced the cytotoxic effect of cisplatin in drug-resistant human gastric cancer cells by inhibition of cyclooxygenase-2 [PubMed: 26407653] |
| PLOS ONE | Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury [PubMed: 26632817] |
| Immunologic Research | Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE9 and is crucial for liver transplant tolerance [PubMed: 25982596] |
| Arquivos Brasileiros de Oftalmologia | Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis [PubMed: 26677041] |
| J Breath Res | Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery [PubMed: 25719610] |
| J Surg Res | Artemisinin inhibits the proliferation, migration, and inflammatory reaction induced by tumor necrosis factor-偽 in vascular smooth muscle cells through nuclear factor kappa B pathway [PubMed: 25575733] |
| Tumor Biology | Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer [Pubmed:27230680] |
| Arthritis Research & Therapy | IL-23/Th17 axis is not influenced by TNF-blocking agents in ankylosing spondylitis patients [Pubmed:26912133] |
| Oncotarget | Metformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axis [Pubmed:27058422] |
| Am J Physiol Gastrointest Liver Physiol | Inhibition of cyclooxygenase-2 alleviates liver cirrhosis via improvement of the dysfunctional Cavia (Guinea pig )t-liver axis in rats [Pubmed:27056726] |
| Oncotarget | Metformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axis. [pubmed:27058422] |
| Lipids. | Dietary Arachidonic Acid Has a Time-Dependent Differential Impact on Adipogenesis Modulated via COX and LOX Pathways in Grass Carp Ctenopharyngodon idellus. [pubmed:27747453] |
| Fish Physiology and Biochemistry | Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid. [pubmed:28012026] |
| Cancer Letters | Metformin inhibits castration-induced EMT in prostate cancer by repressing COX2/PGE2/STAT3 axis. [pubmed:28043910] |
| PLOS?ONE | Identification?of?Biomarkers?of?Impaired?Sensory?Profiles?among?Autistic?Patients. [pubmed:27824861] |
| Scientific Reports | A?Zinc?Morpholine?Complex?Prevents?HCl/Ethanol-Induced?Gastric?Ulcers?in a?Rat?Model. [pubmed:27460157] |
| PLoS One. | Downregulation?of?HOTAIR?Expression?Mediated?Anti-Metastatic?Effect?of?Artesunate?on?CervicalCancer?by?Inhibiting?COX-2?Expression. [pubmed:27736969] |
| Food Funct. | Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways. [pubmed:28256654] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057] |
| Food & Function | Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264. 7 macrophages through the MAPK and NF-κB … [DOI:10.1039/C6FO01873D] |
| Food & Function | Sea cucumber saponins liposomes ameliorate obesity-induced inflammation and insulin resistance in high-fat diet-fed mice [pubmed:29292463] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349] |
| International Journal of Biological Macromolecules | Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage [pubmed:29032210] |
| ONCOLOGY LETTERS | Activation of PGE2/EP2 and PGE2/EP4 signaling pathways positively regulate the level of PD?1 in infiltrating CD8+ T cells in patients with lung cancer [pubmed:29285200] |
| Fish Physiology and Biochemistry | Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid [10.1007/s10695-016-0326-z] |
| Clinical?Cancer?Research | Metformin Inhibits Prostate Cancer Progression by Targeting Tumor-Associated Inflammatory Infiltration [Pubmed:30012567] |
| Biogeography & Macroecology? | Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains [Pubmed:29763701] |
| Scientific Reports | A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling [Pubmed:29523851] |
| Biomedicine & Pharmacotherapy | Steroidal alkaloid solanine A from Linn. exhibits anti-inflammatory activity in lipopolysaccharide/interferon γ-activated murine macrophages and?… [Pubmed:29890469] |
| Inflammopharmacology | Polar extract of protects cartilage homeostasis: possible mechanism of action [Pubmed:29313174] |
| Metabolic?Brain?Disease | Impaired lipid metabolism markers to assess the risk of neuroinflammation in autism spectrum disorder [Pubmed:29569150] |
| Journal of Traditional and Complementary Medicine | Phytochemical evaluation and anti-hemorrhoidal activity of bark of Acacia ferruginea DC [10.1016:j.jtcme.2018.02.003] |
| Cell?Biology?International | Loganin prevents BV‐2 microglia cells from Aβ‐induced inflammation via regulating TLR4/TRAF6/NF‐κB axis [Pubmed: 30288860] |
| Photodiagnosis?and Photodynamic Therapy | Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: Histomorphometrical, Immunohistochemical, Imunological and Microbiological Analyses [Pubmed: 30468900] |
| Biochemical and Biophysical Research Communications | The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion [Pubmed: 30558792] |
| Biological Rhythm Research | Seasonal variations in hormones and enzymes of seminal plasma and its relationship with semen quality in crossbred cattle bulls [Doi: 10.1080/09291016.2018.1548873] |
| Biomedicine & Pharmacotherapy | Resveratrol enhances the protective effects of JBP485 against indomethacin-induced rat intestinal damage in vivo and vitro through up-regulating?… [Pubmed: 30590313] |
| Inflammopharmacology | Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard & [] |
| INTERNATIONAL JOURNAL OF MOLECULAR?SCIENCES? | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| BMC?Pharmacology & Toxicology | Gastroprotective activity of a novel Schiff base derived dibromo substituted compound against ethanol-induced acute gastric lesions in rats [Pubmed: 30770761] |
| Journal of?Cellular?Physiology | Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression [Pubmed: 30671954] |
| Journal of Biological Sciences | Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker [] |
| Journal of Biological Sciences | Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer [] |
| BioMed Research International | Chlorogenic Acid Attenuates Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice through MAPK/ERK/JNK Pathway [] |
| Life?Sciences | 1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway [Pubmed: 30928409] |
| Thoracic?Cancer | Increased expression of TRPV1 in patients with acute or chronic cough after lung cancer surgery [Pubmed: 30883022] |
| Journal of toxicology and environmental health-part a | Melatonin attenuates expression of cyclooxygenase-2 (COX-2) in activated microglia induced by lipopolysaccharide (LPS) [Pubmed: 31081481] |
| Phytotherapy Research | Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammation [Pubmed: 31209984] |
| Food & Function | Antidepressant-like effects of dietary gardenia blue pigment derived from genipin and tyrosine [Doi: 10.1039/C9FO00480G] |
| Food & Function | Synergistic effect of sea cucumber saponin and EPA-enriched phospholipids on insulin resistance in high-fat diet-induced obese mice [Pubmed: 31199413] |
| Biosci Biotechnol Biochem | Polydatin ameliorates chemotherapy-induced cognitive impairment (chemobrain) by inhibiting oxidative stress, inflammatory response, and apoptosis in rats [Pubmed: 31992173] |
| Biomarkers | Management of Citrus sinensis peels for protection and treatment against gastric ulcer induced by ethanol in rats [Pubmed: 32319821] |
| LIFE SCIENCES | Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress [Pubmed: 33227274] |
| Nutrients | Dietary Supplementation of Foxtail Millet Ameliorates Colitis-Associated Colorectal Cancer in Mice via Activation of Gut Receptors and Suppression of the STAT3?… [Pubmed: 32784751] |
| ScientificWorldJournal | The Efficacy of Processing Strategies on the Gastroprotective Potentiality of Chenopodium quinoa Seeds [Pubmed: 32549800] |
| Cell Death & Disease | FADS1 promotes the progression of laryngeal squamous cell carcinoma through activating AKT/mTOR signaling [Pubmed: 32332698] |
| International Journal of Nanomedicine | Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide [Pubmed: 32606669] |
| International Journal of Molecular Medicine | Echinacea polysaccharide attenuates lipopolysaccharide?induced acute kidney injury via inhibiting inflammation, oxidative stress and the MAPK signaling pathway [] |
| Bull Exp Biol Med | Inflammation Mediators Regulate the Microbiota Resistance to Adverse Factors [33222085] |
| Bioengineered | Fenretinide regulates macrophage polarization to protect against experimental colitis induced by dextran sulfate sodium [33380244] |
| Scientific Reports | Bromelain reduced pro-inflammatory mediators as a common pathway that mediate antinociceptive and anti-anxiety effects in sciatic nerve ligated Wistar rats [33432004] |
| J Cell Mol Med | Inhibition of cyclooxygenase?\2 enhanced intestinal epithelial homeostasis via suppressing |??\catenin signalling pathway in experimental liver fibrosis [34145945] |
| Assessment of Galectin-1, Galectin-3, and PGE2 Levels in Patients with COVID-19 [33790073] | |
| Effect of a Proprietary Herbal Product" Arthrocon" on some Synovial Fluid and Serum Biomarkers in Horses with Naturally Occurring Osteoarthritis [] | |
| advances traditional medicine | Induction of apoptosis in activated RAW 264.7 cells and inhibition of pro-inflammatory mediators in rat air pouch by ethylacetate fraction of Ocimum gratissimum leaves [] |
| J Inflamm Res | Novel 1, 3, 4-Oxadiazole Derivatives of Pyrrolo [3, 4-d] Pyridazinone Exert Anti-Inflammatory Activity without Acute Gastrotoxicity in the Carrageenan-Induced?… [34754217] |
| Experimental and Therapeutic Medicine | Anti?inflammation treatment for protection of hepatocytes and amelioration of hepatic fibrosis in rats [34584558] |
| Life Sci | Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus [Pubmed:34990649] |
| J Mol Histol | The ameliorative effects of cinnamon oil against ethanol-induced gastric ulcer in rats by regulating oxidative stress and promoting angiogenesis [Pubmed:35290563] |
| Cell Signal | Suppression of COX-2/PGE2 levels by carbazole-linked triazoles via modulating methylglyoxal-AGEs and glucose-AGEs–Induced ROS/NF-κB signaling in monocytes [Pubmed:35640822] |
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