Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)
P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
- 編號UCEA957Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍370.4-30,000pg/mL
- 靈敏度最小可檢測劑量小于等于138.2pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1392 ¥ 1989 ¥ 8951 ¥ 16907 ¥ 139230
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測Ⅰ型前膠原氨基端原肽(PINP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型前膠原氨基端原肽(PINP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-104 | 95 |
| EDTA plasma(n=5) | 94-103 | 99 |
| heparin plasma(n=5) | 83-92 | 86 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型前膠原氨基端原肽(PINP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅰ型前膠原氨基端原肽(PINP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 90-104% | 99-105% | 99-105% |
| EDTA plasma(n=5) | 78-105% | 95-102% | 78-88% | 82-91% |
| heparin plasma(n=5) | 88-96% | 93-102% | 90-97% | 80-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA957Mu01 | Ⅰ型前膠原氨基端原肽(PINP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| USPA957Mu01 | Ⅰ型前膠原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
| UCPA957Mu21 | Ⅰ型前膠原氨基端原肽(PINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA957Mu02 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB |
| UPAA957Mu01 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA957Mu08 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | Testing in progress |
| UMAA957Mu21 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | ICC/IF |
| USEA957Mu | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA957Mu | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA957Mu | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA957Mu | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA957Mu | Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA957Mu11 | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Trials | Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. [Trials: 1745-6215] |
| Clinical Biochemistry | Measurement of aminoterminal propeptide of type I procollagen (PINP) in serum [ScienceDirect: S0009912012001543] |
| Therapeutic Apheresis and Dialysis | Low molecular weight iron dextran increases fibroblast growth factor-23 concentration, together with parathyroid hormone decrease in hemodialyzed patients [PubMed: 22458393] |
| International Journal of Clinical & Experimental Pathology | IL4 and IL-17A provide a Th2/Th17-polarized inflammatory milieu in favor of TGF-β1 to induce bronchial epithelial-mesenchymal transition (EMT) [PubMed: PMC3726963] |
| Osteoporosis International | PINP as a biological response marker during teriparatide treatment for osteoporosis [Pubmed: 24599274] |
| Clinical Cancer Research | Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo [Aacrjournals: Source] |
| Endocrinology | Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain [Endocrine: Source] |
| general endocrinology | Oxytocin reverse ovariectomy-induced Osteoper and body fat gain [Pubmed:24506069] |
| J Neurol Sci. | The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. [Pubmed:24629477] |
| Bone. | Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models [Pubmed:24947449] |
| Journal of Materials Science: Materials in Medicine | Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis [Pubmed:24980874] |
| Springerplus. | Effects of de-escalated bisphosphonate therapy on bone turnover biomarkers in breast cancer patients with bone metastases [Pubmed:25332877] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861] |
| frontiers in endocrinology | Oxytocin reverses osteoporosis in a sex-dependent manner [PubMed: 26042090] |
| MERIT RESEARCH JOURNALS | Changes in Bone Metabolism Months after Laparoscopic Morbidly Obese Pre-menopausal Women [Mms: Content] |
| Heart Rhythm.? | Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population [Pubmed:26776557] |
| BMJ Open Sport Exerc Med | Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupture [content:2] |
| Bibliothekssystem Universit?t Hamburg | Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten [volltexte:2016] |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
| Endocrine | Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners [pubmed:27422791] |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
| journal of science and medicine in sport | The effect of calcium and vitamin D supplementation on bone health of male Jockeys. [pubmed:27568072] |
| Combined aerobic and resistance training improves bone health of female cancer survivors [S2352187216300389] | |
| Cellular?Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy [pubmed:27889753] |
| Epilepsy?Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats [pubmed:27838501] |
| Sci Rep.? | Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. [pubmed:28303909] |
| Metabolism.? | Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. [pubmed:28521873] |
| Heart and vessels | Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillation [pubmed:28314975] |
| SCIENCE & TECHNOLOGY | A Study Protocol: Spinal Morphology, Physical Performance, Quality of Life and Biochemical Markers in Adults at Risk of Osteoporotic Fractures [59f7c85aaca272607e2d8d7a] |
| Knee Surg Sports Traumatol Arthrosc | Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis [pubmed:28668970] |
| Method for the treatment of cardiovascular fibrosis [patents:US9594083] | |
| Clinical Cases in Mineral and Bone Metabolism | Periostin and sclerostin levels in juvenile Paget's disease [pubmed:29263750] |
| Journal of Clinical Research in Pediatric Endocrinology | Congenital Hypothyroidism and Bone Remodeling Cycle [pubmed:27840329] |
| Biomarkers Medicial | Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus [pubmed:28685602] |
| Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy?detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
| BioMed Research International | Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research [pubmed:29119115] |
| Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
| Bone. | The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patients [pubmed:29129760] |
| Cell?Death?And Differentiation | TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 [Pubmed:29358671] |
| Nutrients | Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women—A Randomized Controlled Study [Pubmed:29337906] |
| Osteoporosis International | Two-year cortical and trabecular bone loss in CKD-5D: biochemical and clinical predictors [Pubmed:28993865] |
| Journal of Nutrition | Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D Deficiency [Pubmed:29897564] |
| European?Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism [Pubmed:29428468] |
| Journal of Clinical Densitometry | Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study [Pubmed:30076009] |
| Hernia | Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia [Pubmed:29484522] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
| Nature | Coupling of bone resorption and formation by RANKL reverse signalling [Pubmed: 30185903] |
| European?Journal of?Medical?Research | Biomechanical and histological analyses of the fracture healing process after direct or prolonged reduction [Pubmed: 30180907] |
| Sensors | Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers [Pubmed: 30181433] |
| Trials | Structured diet and exercise guidance in pregnancy to improve health in women and their offspring: study protocol for the Be Healthy in Pregnancy (BHIP)?… [Pubmed: 30567604] |
| International Journal of Cancer | Effect of induction therapy with lenalidomide, doxorubicin, and dexamethasone on bone remodeling and angiogenesis in newly diagnosed multiple myeloma [Pubmed: 30650184] |
| Bone | Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis [Pubmed: 30610968] |
| Revista de Chimie -Bucharest- | Quantification of N-Terminal Procollagen I Propeptide in Patients With Occlusal Trauma Induced by Prosthetic Factors [] |
| PLOS?ONE | Effect of antibiotic infused calcium sulfate/hydroxyapatite (CAS/HA) insets on implant-associated osteitis in a femur fracture model in mice [Pubmed: 30870491] |
| Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
| Current Developments in Nutrition | Changes in Calciotropic Hormones and Bone Markers During Pregnancy in Response to a Nutrition+ Exercise Intervention in the Be Healthy in Pregnancy RCT (P11?… [Pubmed: 31224444] |
| Stem Cell Research & Therapy | Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB?… [Pubmed: 31324207] |
| Bone | The long noncoding RNA Crnde regulates osteoblast proliferation through the Wnt/β-catenin signaling pathway in mice [Pubmed: 31622775] |
| International Journal of Basic and Clinical Endocrinology | Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940] |
| Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition [Pubmed: 31678489] |
| International journal of molecular sciences | Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients [Pubmed: 31717649] |
| Journal of Bone and Mineral Research | BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling [Pubmed: 31914211] |
| ESC Heart Failure | Outcome in German and South African peripartum cardiomyopathy cohorts associates with medical therapy and fibrosis markers [Pubmed: 32064780] |
| International Journal of Medical Sciences | Hypoalbuminemia differently affects the serum bone turnover markers in hemodialysis patients [Pubmed: 31839746] |
| JOURNAL OF BONE AND MINERAL RESEARCH | Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone Formation [Pubmed: 33180356] |
| JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | Development of a novel polysaccharide-based iron oxide nanoparticle to prevent iron accumulation-related osteoporosis by scavenging reactive oxygen?… [Pubmed: 33049237] |
| journal of bone and mineral research | Mice carrying a ubiquitous R235W mutation of Wnt1 display a bone‐specific phenotype [Pubmed: 32369212] |
| Endokrynologia Polska | FGF23 and primary hyperparathyroidism: is there a link? [Pubmed: 32598019] |
| Journal of Orthopaedic Translation | Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats [] |
| Research square | 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice [] |
| 淫羊藿水提物及提取藥渣的抗骨質(zhì)疏松藥效比較研究 [] | |
| Pamukkale T?p Dergisi | Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza ba?l? k?r?k riskinin ve kemik Turnoveri'nin de?erlendirilmesi [] |
| International Journal of Pharmaceutical and Clinical Research | Bone Turnover Markers in Osteogenesis Imperfecta and Effect of Bisphosphonate Treatment: First Egyptian Study [] |
| Science Advances | A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair [33310848] |
| Rheumatology | Proinflammatory and bone protective role of calcitonin gene-related peptide alpha in collagen antibody-induced arthritis [33221885] |
| Nutrients | Low Energy Availability with and without a High-Protein Diet Suppresses Bone Formation and Increases Bone Resorption in Men: A Randomized Controlled Pilot ?- [33671093] |
| J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/|??\catenin pathway [33950533] |
| Phytomedicine | Randomized, double-blind, placebo-controlled trial to examine the safety, pharmacokinetics and effects of Epimedium prenylflavonoids, on bone specific alkaline?… [34352588] |
| Nutrients | The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized?… [34684621] |
| PLoS One | Osteoblast-specific inactivation of p53 results in locally increased bone formation [34793446] |
| Bone Res | The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV [34759273] |
| Nutrients | The Effect of Caloric Restriction with and without n-3 PUFA Supplementation on Bone Turnover Markers in Blood of Subjects with Abdominal Obesity: A Randomized?… [34578973] |
| Chronobiol Int | Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in Poland [Pubmed:35139709] |
| Cancers (Basel) | Spine Metastases in Immunocompromised Mice after Intracardiac Injection of MDA-MB-231-SCP2 Breast Cancer Cells [Pubmed:35158823] |
| Cell Death Discov | BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis [Pubmed:35523787] |
| Experimental and Molecular Medicine | Foxf2 represses bone formation via Wnt2b/β-catenin signaling [Pubmed:35668101] |
| Bioactive Materials | Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles [Pubmed:35386445] |
| Journal of Orthopaedic Translation | Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathway [Pubmed:35228997] |
| Bioactive Materials | Construction of a magnesium hydroxide/graphene oxide/hydroxyapatite composite coating on Mg–Ca–Zn–Ag alloy to inhibit bacterial infection and promote?… [Pubmed:35585377] |
| Journal of Orthopaedic Translation | Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35?… [] |
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