正五聚蛋白3(PTX3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Pentraxin 3, Long (PTX3)
TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein
- 編號USEK411Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍31.2-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于13.6pg/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1596 ¥ 2280 ¥ 10260 ¥ 19380 ¥ 159600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測正五聚蛋白3(PTX3)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的正五聚蛋白3(PTX3)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-98 | 82 |
| EDTA plasma(n=5) | 90-104 | 98 |
| heparin plasma(n=5) | 81-89 | 85 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的正五聚蛋白3(PTX3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中正五聚蛋白3(PTX3)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-94% | 95-102% | 98-105% | 83-96% |
| EDTA plasma(n=5) | 82-103% | 86-105% | 79-103% | 94-105% |
| heparin plasma(n=5) | 81-99% | 88-102% | 84-105% | 84-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將正五聚蛋白3(PTX3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的正五聚蛋白3(PTX3)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的正五聚蛋白3(PTX3)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的正五聚蛋白3(PTX3)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPK411Ra61 | 正五聚蛋白3(PTX3)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPK411Ra01 | 正五聚蛋白3(PTX3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAK411Ra01 | 正五聚蛋白3(PTX3)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAK411Ra21 | 正五聚蛋白3(PTX3)單克隆抗體 | WB; IHC; ICC; IP. |
| USEK411Ra | 正五聚蛋白3(PTX3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMK411Ra | 正五聚蛋白3(PTX3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Mediators of Inflammation | Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model [PubMed: 22966213] |
| Ibnosina Journal of Medicine and Biomedical Sciences | Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand Rabbits [Sfu: Source] |
| Hemodialysis International | Hemodialysis-induced changes in the blood composition affect function of the endothelium [Onlinelibrary: hdi.12148] |
| Hemodial Int. | Hemodialysis‐induced changes in the blood composition affect function of the endothelium [Pubmed:24612443] |
| Journal of Obstetrics and Gynaecology Research | The effects of sunitinib on endometriosis [Pubmed:25093747] |
| Gynecol Endocrinol. | Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic rats [Pubmed:25366063] |
| Taiwanese Journal of Obstetrics and Gynecology | Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3 [Pubmed:25510691] |
| Journal of Obstetrics and Gynaecology Research | Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parameters [PubMed: 26223286] |
| J Obstet Gynaecol | The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat model [PubMed: 26470829] |
| Neurology Research International | Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers [5952149] |
| Journal of?Diabetes?Research | Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat Model [Pubmed:29725602] |
| World Journal of Cardiovascular Diseases | The Relationship between Serum Pentraxin-3 Levels and Severity of Coronary Heart Disease [paperID:86206] |
| Advances in Medical Sciences | Serum levels and gene expression of pentraxin 3 are elevated in COPD [Pubmed: 30572222] |
| BioMed Research International | Pentraxin 3 Detects Clinically Significant Fibrosis in Patients with Chronic Viral Hepatitis C [] |
| Evidence-Based Complementary and Alternative Medicine | Jie Du Tong Ye San Prevents N-Nitrosomethylbenzylamine-Induced Esophageal Carcinogenesis via Inhibition of Inflammation and Proliferation [] |
| Journal of Interferon and Cytokine Research | Study on Serum Pentraxin-3 Levels in Vasculitis with Hypertension [Pubmed: 31264920] |
| Scientific Reports | The role of CRP and Pentraxin 3 in the prediction of systemic inflammatory response syndrome and death in acute pancreatitis [Pubmed: 31798002] |
| J Obstet Gynaecol | Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental study [Pubmed: 31955629] |
| J Pediatr Adolesc Gynecol | Effects of Methylene Blue on Ovarian TorsionLDetorsion Injury in a Rat Model [Pubmed: 32593749] |
| Acta Cirurgica Brasileira | Therapeutic effects of vitamin D on acetic acid-induced colitis in rats [Pubmed: 32555936] |
| Acta Cardiol Sin | Pentraxin 3 as a marker for cardiovascular disease risk in overweight and obese children [33716459] |
| International Journal of Endocrinology | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet??- [34035808] |
| Nutrients | High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese?… [34684637] |
| J Obstet Gynaecol Res | Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus [34595798] |
