對氧磷酶1(PON1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Paraoxonase 1 (PON1)
ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
- 編號USEA243Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍125-8,000ng/mL
- 靈敏度最小可檢測劑量小于等于49ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1361 ¥ 1944 ¥ 8748 ¥ 16524 ¥ 136080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測對氧磷酶1(PON1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的對氧磷酶1(PON1)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-98 | 92 |
| EDTA plasma(n=5) | 84-101 | 94 |
| heparin plasma(n=5) | 95-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的對氧磷酶1(PON1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中對氧磷酶1(PON1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-88% | 89-98% | 80-90% | 91-101% |
| EDTA plasma(n=5) | 91-99% | 95-103% | 84-95% | 82-103% |
| heparin plasma(n=5) | 87-101% | 94-101% | 84-98% | 81-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將對氧磷酶1(PON1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的對氧磷酶1(PON1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的對氧磷酶1(PON1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的對氧磷酶1(PON1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA243Mu01 | 對氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA243Mu02 | 對氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA243Mu61 | 對氧磷酶1(PON1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA243Mu01 | 對氧磷酶1(PON1)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA243Mu02 | 對氧磷酶1(PON1)多克隆抗體 | WB; IHC; ICC/IF |
| ULAA243Mu71 | 對氧磷酶1(PON1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA243Mu | 對氧磷酶1(PON1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA243Mu | 對氧磷酶1(PON1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA243Mu01 | 對氧磷酶1(PON1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| British Journal of Nutrition | Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activities [Pubmed: 23510814] |
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| Clinical and Experimental Hypertension | Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension [pubmed:27668323] |
| Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian population [pubmed:29254890] |
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| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
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| American Journal of Food Science and Health | Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats [ISSN: 2381-7224] |
| Metabolic brain disease | Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels [pubmed:28808839] |
| Therapeutic Apheresis And Dialysis | Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease [Pubmed:29368426] |
| Biological?Trace?Element?Research | Effect of Aqueous Extract of?Glycyrrhiza glabra?on the Biochemical Changes Induced by Cadmium Chloride in Rats [Pubmed: 30251228] |
| GLYCOCONJUGATE JOURNAL | Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer [Pubmed: 30607521] |
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| Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828] |
| Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605] |
| Nutrition, Metabolism and Cardiovascular Diseases | Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake [Pubmed: 31757567] |
| Environment International | Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins [Pubmed: 31783242] |
| RESPIRATORY RESEARCH | The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation?… [Pubmed: 33148285] |
| CLINICAL RHEUMATOLOGY | PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients [Pubmed: 32556934] |
| respiratory research | The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction [] |
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| Oxid Med Cell Longev | Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men [34917230] |
