基質(zhì)細胞衍生因子1(SDF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)
CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
- 編號USEA122Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍62.5-4,000pg/mL
- 靈敏度最小可檢測劑量小于等于25.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1361 ¥ 1944 ¥ 8748 ¥ 16524 ¥ 136080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測基質(zhì)細胞衍生因子1(SDF1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的基質(zhì)細胞衍生因子1(SDF1)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 82-97 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)細胞衍生因子1(SDF1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中基質(zhì)細胞衍生因子1(SDF1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-88% | 91-102% | 88-95% | 83-101% |
| EDTA plasma(n=5) | 81-93% | 97-104% | 96-103% | 85-94% |
| heparin plasma(n=5) | 95-105% | 82-101% | 95-105% | 78-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將基質(zhì)細胞衍生因子1(SDF1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的基質(zhì)細胞衍生因子1(SDF1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的基質(zhì)細胞衍生因子1(SDF1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的基質(zhì)細胞衍生因子1(SDF1)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA122Mu02 | 基質(zhì)細胞衍生因子1(SDF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA122Mu01 | 基質(zhì)細胞衍生因子1(SDF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA122Mu02 | 基質(zhì)細胞衍生因子1(SDF1)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA122Mu01 | 基質(zhì)細胞衍生因子1(SDF1)多克隆抗體 | IHC |
| ULAA122Mu71 | 基質(zhì)細胞衍生因子1(SDF1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| ULAA122Mu81 | 基質(zhì)細胞衍生因子1(SDF1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMEA122Mu | 基質(zhì)細胞衍生因子1(SDF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA122Mu | 基質(zhì)細胞衍生因子1(SDF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA122Mu | 基質(zhì)細胞衍生因子1(SDF1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Critical Care | Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD) [Goescholar: source] |
| 22 | The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels [ScienceDirect: S0142961209012654] |
| 22 | The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms [ScienceDirect: S0142961211011422] |
| Phytomedicine | Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model [ScienceDirect: S0944711310003260] |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells [PubMed: 21655971] |
| Molecular Therapy | Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia [PubMed: 21224834] |
| Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis [PubMed: 22797069] |
| International Journal of Nephrology | Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis [PubMed: 22792461] |
| Rheumatology International | Serum levels of selected chemokines in systemic lupus erythematosus patients [Pubmed: 22461186] |
| Experimental Eye Research | Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells [Pubmed: 24780853] |
| Atherosclerosis | The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial [Pubmed:24530788] |
| International Journal of Cardiology | Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men [Pubmed:24286591] |
| Tissue Engineering Part A | Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat [Pubmed:24814095] |
| Pharmacol Rep.? | Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression [Pubmed:25150002] |
| Journal of Stroke and Cerebrovascular Diseases | Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients [Pubmed:25444027] |
| Pharmacological Reports | The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders [Pubmed:25560580] |
| In Vitro Cellular & Developmental Biology - Animal | Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells [Pubmed:25636237] |
| Pharmacological Reports | Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder [PubMed: 26481549] |
| Biochem Biophys Res Commun | Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats [PubMed: 26392311] |
| Am J Transl Res | Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats [PubMed: 26396666] |
| Medicine (Baltimore) | A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion [PubMed: 26632727] |
| Pharmacological Reports | A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity [Pubmed:27208878] |
| Frontiers in Psychiatry | Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. [pubmed:28149283] |
| European?Journal?of?Applied?Physiology | High-intensity?Interval?training?enhances?mobilization/functionality?of?endothelial?progenitor?cellsand?depressed?shedding?of?vascular?endothelial?cells?undergoing hypoxia. [pubmed:27761657] |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs [pubmed:29233725] |
| International Journal of?Clinical?and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens [ISSN:1936-2625/IJCEP0058424] |
| BioMed Research International. | c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer [pubmed:28573141] |
| Scientific Reports | Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms. [pubmed:28842629] |
| Biomaterials | Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study. [pubmed:29017079] |
| Biomaterials?Science | The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration [Pubmed:29850672] |
| International Journal of Molecular?Medicine | Low?intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1?mediated SDF?1 expression [Pubmed:29620151] |
| Cardiovascular Drugs and Therapy | β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive rats [Pubmed:29752864] |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation [Doi: 10.1016/j.jcyt.2018.07.007] |
| ACS?Applied?Materials?&?Interfaces | Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone Regeneration [Pubmed: 30777748] |
| World?Journal of?Stem?Cells | Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cells [Pubmed: 30949297] |
| Stem Cells International | Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells [Pubmed: 31827537] |
| Stem Cell Res Ther | Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus [Pubmed: 32894200] |
| Journal of tissue engineering and regenerative medicine | Enhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4 [Pubmed: 32633015] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| pharmaceuticals | bFGF and SDF-1|á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts [33800631] |
| Microb Biotechnol | Combination of an engineered Lactococcus?lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice [34310856] |
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