腎損傷分子1(Kim1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Kidney Injury Molecule 1 (Kim1)
HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1
- 編號USEA785Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍39-2,500pg/mL
- 靈敏度最小可檢測劑量小于等于14pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 983 ¥ 1404 ¥ 6318 ¥ 11934 ¥ 98280
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測腎損傷分子1(Kim1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的腎損傷分子1(Kim1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-102 | 97 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 78-102 | 92 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的腎損傷分子1(Kim1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腎損傷分子1(Kim1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-102% | 80-105% | 85-92% | 80-90% |
| EDTA plasma(n=5) | 93-104% | 80-95% | 89-98% | 90-97% |
| heparin plasma(n=5) | 88-101% | 90-97% | 89-99% | 81-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將腎損傷分子1(Kim1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的腎損傷分子1(Kim1)與連接于固相載體上的抗體結合，然后加入生物素化的腎損傷分子1(Kim1)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的腎損傷分子1(Kim1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA785Mu01 | 腎損傷分子1(Kim1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA785Mu02 | 腎損傷分子1(Kim1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA785Mu61 | 腎損傷分子1(Kim1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA785Mu61 | 腎損傷分子1(Kim1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA785Mu01 | 腎損傷分子1(Kim1)多克隆抗體 | WB; ICC/IF |
| UPAA785Mu02 | 腎損傷分子1(Kim1)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA785Mu81 | 腎損傷分子1(Kim1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA785Mu71 | 腎損傷分子1(Kim1)多克隆抗體(生物素標記) | WB |
| USEA785Mu | 腎損傷分子1(Kim1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA785Mu | 腎損傷分子1(Kim1)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA785Mu | 腎損傷分子1(Kim1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA785Mu01 | 腎損傷分子1(Kim1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Clinical Toxicology | Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication [PubMed: 19827907] |
| Pediatric Nephrology | KIM-1 and NGAL: new markers of obstructive nephropathy [SpringerLink: v461592787w5488x] |
| Pediatric Research | Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight Infants [LWW: 10000] |
| Archives of Medical Science | Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rate [AMS: source] |
| Nephrology | Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content [PubMed: 23046363] |
| Nanotoxicology. | Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO(2)) nanoparticles in rats. [PubMed: 23272807] |
| Toxicological Science | A Mouse Diversity Panel Approach Reveals the Potential for Clinical Kidney Injury Due to DB289 Not Predicted by Classical Rodent Models [PubMed: PMC3498743] |
| Disease Markers | Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer Treatment [Hindawi: 369784] |
| Journal of Biomarkers | Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model [Hindawi: 413853] |
| Advances in Life Science and Technology | Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury. [Iiste: Source] |
| Nephrology | Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant [Pubmed: 24325180] |
| Nephrology | Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content. [Pubmed: 23046363] |
| BioMed Research International | The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies [Hindawi: 740105] |
| Life Sci. | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model [Pubmed:25476829] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source] |
| 臨床兒科雜志 | 中性粒細胞明膠酶相關脂質(zhì)運載蛋白、腎損傷分子1和白介素18 在診斷心肺分流術后急性腎損傷中的意義 [Jcp-Sh:Source] |
| Nephrology (Carlton). | Tubular urinary biomarkers do not identify aetiology of acute kidney injury in kidney transplant recipients [Pubmed:24666430] |
| PLoS One. | Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434] |
| BMC Nephrology | SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trial [Pubmed:24996842] |
| Pediatric Nephrology | The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkers [Pubmed:25127917] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
| Transworld Medical Journal | Kidney injury molecule-1: A urinary biomarker for contrast-induced acute kidney injury [Tmjournals:Source] |
| Int J Clin Exp Pathol | Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis [PubMed: 26191125] |
| Int J Clin Exp Pathol | Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice [PubMed: 26045773] |
| Mol Med Rep | Vorinostat protects against calcium oxalate-induced kidney injury in mice [PubMed: 26095064] |
| Pediatr Nephrol | Role of new biomarkers for predicting renal scarring in vesicoureteral reflux: NGAL, KIM-1, and L-FABP [PubMed: 26324091] |
| Journal of Pediatric Urology | Urinary NGAL, KIM-1 and L-FABP concentrations in antenatal hydronephrosis [PubMed: 26096437] |
| Am J Physiol Renal Physiol | Intrarenal Renin-Angiotensin System Mediates Fatty Acid-Induced ER Stress in the Kidney [PubMed: 26672616] |
| Mol Med Rep. | Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti?inflammatory, and anti?apoptotic activities. [Pubmed:27082705] |
| Nephrol. Dial. Transplant. | Preconditioned suppression of prolyl-hydroxylases attenuates renal injury but increases mortality in septic murine models [Pubmed:26908768] |
| J Clin Lab Anal.? | Kidney Injury Molecule Levels in Type 2 Diabetes Mellitus [Pubmed:27132791] |
| Med Sci Monit. | Protective Effect of Dihydromyricetin Against Lipopolysaccharide-Induced Acute Kidney Injury in a Rat Model [pmc:PMC4754091] |
| Diabetes. | Deficiency in Apoptosis-Inducing Factor Recapitulates Chronic Kidney Disease via Aberrant Mitochondrial Homeostasis [Pubmed:26822084] |
| Sci Rep. | Once daily administration of the SGLT2 inhibitor, empagliflozin, attenuates markers of renal fibrosis without improving albuminuria in diabetic db/db mice [Pubmed:27226136] |
| Journal of Applied Life Sciences International | Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in Rats [Barakat812016JALSI28698.pdf] |
| Oxidative Medicine and Cellular Longevity | Synergic Effect of α-Mangostin on the Cytotoxicity of Cisplatin in a Cervical Cancer Model. [pubmed:28053694] |
| Molecular Medicine Reports | Attenuation?of?renal?ischemia/reperfusion?injury?by?oleanolic acid?preconditioning?via?itsantioxidant, anti?inflammatory, and anti?apoptotic?activities. [pubmed:27082705] |
| Journal of Agricultural and Food Chemistry | Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Mice [pubmed:29254330] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. [pubmed:29027259] |
| Nephron - Physiology | Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise [10.1159/000481179] |
| Cellular?Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats?… [Pubmed:29550811] |
| Frontiers in Pharmacology | Exploring the therapeutic mechanism of Desmodium styracifolium on oxalate crystal-induced kidney injuries using comprehensive approaches based on?… [Pubmed:29950996] |
| Molecules | Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy [Pubmed:29642511] |
| American Journal of Physiology-Regulatory Integrative and Comparative Physiology | Modeling heart failure risk in diabetes and kidney disease: limitations and potential applications of transverse aortic constriction in high-fat-fed mice [Pubmed:29443547] |
| Biological?Trace?Element?Research | Effect of Aqueous Extract of?Glycyrrhiza glabra?on the Biochemical Changes Induced by Cadmium Chloride in Rats [Pubmed: 30251228] |
| Cancer?Research?and?Treatment | A Phage Display-Identified Peptide Selectively Binds to Kidney Injury Molecule-1(KIM-1) and Detects KIM-1-Overexpressing Tumors in vivo [] |
| Archives?of?Medical?Science | Urinary neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, N-acetyl-β-D-glucosaminidase levels and mortality risk in septic patients with?… [Pubmed: 30393493] |
| Biological?&?Pharmaceutical?Bulletin | UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury [Pubmed: 30381664] |
| Kidney?and?Blood?Pressure?Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713] |
| Clinica?Chimica?Acta | Urine markers of renal tubular injury in idiopathic membranous nephropathy: A cross sectional study [Pubmed: 30684459] |
| BMC?Complementary and Alternative Medicine | Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine [Pubmed: 30871515] |
| 2.583 | Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors [Doi: BioMed Research International] |
| Naunyn-Schmiedeberg's archives of pharmacology | Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade [Pubmed: 31197431] |
| Egyptian Journal of Zoology | Effect of Cucumis melo var. flexuosus leaves extract on renal oxidative injury and inflammation in diabetic male albino rats [] |
| Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605] |
| Bioscience Reports | page Metabolomics study of fasudil on cisplatin-induced kidney injury [Pubmed: 31670380] |
| life sciences | MiR-214-3p exacerbates kidney damages and inflammation induced by hyperlipidemic pancreatitis complicated with acute renal injury [Pubmed: 31790686] |
| Arch Physiol Biochem | Protective effect of saxagliptin against renal ischaemia reperfusion injury in rats [Pubmed: 31979992] |
| clinical science | Delineating a role for the Mitochondrial Permeability Transition Pore in Diabetic Kidney Disease by targeting Cyclophilin D [Pubmed: 31943002] |
| LIFE SCIENCES | Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway [Pubmed: 32798557] |
| INTERNATIONAL UROLOGY AND NEPHROLOGY | Serum NGAL, KIM-1, IL-18, L-FABP: new biomarkers in the diagnostics of acute kidney injury (AKI) following invasive cardiology procedures [Pubmed: 32557377] |
| Journal of Pediatric Urology | Can Serum Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Help in Decision Making for Surgery in Antenatally Dedected?… [Pubmed: 33139211] |
| Ratlarda sodyum florid ile olu?turulan toksikasyonda Karvakol'ün b?brekler üzerine etkisi [] | |
| Rationale and design of the intervention in PHOspholamban RElated CArdiomyopathy STudy (i-PHORECAST) [] | |
| Pediatr Int | Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers [33423326] |
| J Int Med Res | Value of urinary kidney injury molecule-1 levels in predicting acute kidney injury in very low birth weight preterm infants [33372811] |
| Biomed Res Int | Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury [33604384] |
| Nutrients | Targeting Methylglyoxal in Diabetic Kidney Disease Using the Mitochondria-Targeted Compound MitoGamide [33922959] |
| J Am Soc Nephrol | Kim-1 Targeted Extracellular Vesicles: A New Therapeutic Platform for RNAi to Treat AKI [34127536] |
| Heliyon | Anti-oxidant impact of Lisinopril and Enalapril against acute kidney injury induced by doxorubicin in male Wistar rats: involvement of kidney injury molecule-1 [33506137] |
| Journal of Pharmacological Sciences | Pentoxifylline treatment alleviates kidney ischemia/reperfusion injury: Novel involvement of galectin-3 and ASK-1/JNK & ERK1/2/NF-|êB/HMGB-1 trajectories [34030796] |
| Int Urol Nephrol | Could urinary kidney injury molecule-1 be a good marker in subclinical acute kidney injury in mild to moderate COVID-19 infection? [34213713] |
| Intensive Care Medicine Experimental | Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental?… [34169407] |
| iscience | Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure [34820603] |
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