核因子κB受體激活因子配體(RANkL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine
- 編號USEA855Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍3.12-200pg/mL
- 靈敏度最小可檢測劑量小于等于1.27pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1323 ¥ 1890 ¥ 8505 ¥ 16065 ¥ 132300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測核因子κB受體激活因子配體(RANkL)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的核因子κB受體激活因子配體(RANkL)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-99 | 94 |
| EDTA plasma(n=5) | 88-101 | 97 |
| heparin plasma(n=5) | 98-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的核因子κB受體激活因子配體(RANkL)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中核因子κB受體激活因子配體(RANkL)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-97% | 87-96% | 91-105% | 97-105% |
| EDTA plasma(n=5) | 80-103% | 89-96% | 97-105% | 80-101% |
| heparin plasma(n=5) | 78-94% | 84-104% | 91-98% | 90-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將核因子κB受體激活因子配體(RANkL)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的核因子κB受體激活因子配體(RANkL)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的核因子κB受體激活因子配體(RANkL)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的核因子κB受體激活因子配體(RANkL)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA855Hu02 | 核因子κB受體激活因子配體(RANkL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA855Hu01 | 核因子κB受體激活因子配體(RANkL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA855Hu01 | 核因子κB受體激活因子配體(RANkL)多克隆抗體 | WB |
| URAA855Hu21 | 核因子κB受體激活因子配體(RANkL)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA855Hu21 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB |
| UMAA855Hu22 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| UFAA855Hu02 | 抗核因子κB受體激活因子配體(RANkL)單克隆抗體 | FCM |
| ULAA855Hu72 | 核因子κB受體激活因子配體(RANkL)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA855Hu | 核因子κB受體激活因子配體(RANkL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA855Hu | 核因子κB受體激活因子配體(RANkL)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA855Hu | 核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA855Hu01 | 核因子κB受體激活因子配體(RANkL)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA855Hu01 | 核因子κB受體激活因子配體(RANkL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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