鐵蛋白(FE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Ferritin (FE)
- 編號(hào)USEA518Ga
- 物種Chicken (Gallus，雞) 相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍6.25-400ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于2.24ng/mL.
- 樣本類(lèi)型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷(xiāo)商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)鐵蛋白(FE)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的鐵蛋白(FE)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-97 | 94 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 92-101 | 95 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的鐵蛋白(FE)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中鐵蛋白(FE)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-99% | 81-95% | 95-105% | 91-99% |
| EDTA plasma(n=5) | 98-105% | 85-93% | 80-105% | 81-101% |
| heparin plasma(n=5) | 80-101% | 85-99% | 86-101% | 94-101% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將鐵蛋白(FE)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的鐵蛋白(FE)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的鐵蛋白(FE)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的鐵蛋白(FE)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| USEA518Ga | 鐵蛋白(FE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA518Ga | 鐵蛋白(FE)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular Endocrinology | PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. [Pubmed: 23438894] |
| experimental oncology | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. [PubMed: 25804229] |
| J Cancer Sci Ther | Host H68D Genotype Affects Tumor Growth in Mouse Melanoma [Open-Access: 1948-5956-1000353] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699] |
| Physiological research | The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743] |
| Expert?Review?of Hematology | 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. [pubmed:27615544] |
| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463] |
| European Journal of Pharmaceutical Sciences | sucrosomial? iron absorption studied by in vitro and ex-vivo models [pubmed:29055735] |
| Journal of Food Science and Technology | In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. [pubmed:29051659] |
| International Journal of Cuban Health & Medicine | Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women [10.1590/MEDICC.2017.1902030004?] |
| Oman Medical Journal | The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599] |
| Food Bioscience | Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system [10.1016/j.fbio.2017.10.001] |
| Nutrients. | Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats [pubmed:28629118] |
| Food?Chemistry | Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells [Pubmed:29622185] |
| Animal Feed Science and Technology | Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet [10.1016:j.anifeedsci.2018.01.012] |
| American Journal of Hematology | Iron homeostasis in pregnancy and spontaneous abortion [Pubmed: 30394565] |
| The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria | Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria [] |
| Haematologica | New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208] |
| 博士論文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
| Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic [] | |
| Cell Research | Transferrin plays a central role in coagulation balance by interacting with clotting factors [Pubmed: 31811276] |
| international journal of biological macromolecules | Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications [Pubmed: 31904465] |
| journal of the american college of nutrition | Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation [Pubmed: 32255407] |
| LWT-FOOD SCIENCE AND TECHNOLOGY | Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability [] |
| J Mol Cell Cardiol | Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233] |
| European Journal of Pharmacology | The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 [Pubmed:35700835] |
