生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Growth Hormone (GH)
GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal
- 編號UCEA044Eq
- 物種Equus caballus; Equine (Horse，馬) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍246.9-20,000pg/mL
- 靈敏度最小可檢測劑量小于等于91.3pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1625 ¥ 2322 ¥ 10449 ¥ 19737 ¥ 162540
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測生長激素(GH)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質水平。將生長激素(GH)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經(jīng)洗滌去掉未結合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Equus caballus; Equine (Horse，馬) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA044Eq01 | 生長激素(GH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA044Eq01 | 生長激素(GH)多克隆抗體 | WB |
| UMAA044Eq21 | 生長激素(GH)單克隆抗體 | WB; IHC; ICC; IP. |
| UCEA044Eq | 生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA044Eq | 生長激素(GH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Veterinary Recprd Case Report | Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism [Bmj: Source] |
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| Journal of Animal Science | Dietary lysine affects amino acid metabolism and growth performance, which may not involve the GH/IGF-1 axis, in young growing pigs [Pubmed: 31922564] |
| SAUDI MEDICAL JOURNAL | The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additive [Pubmed: 31287125] |
| Frontiers in Endocrinology | RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034] |
| JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | Isolated growth hormone deficiency in a Chihuahua with a GH1 mutation [Pubmed: 32646299] |
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| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling [33492754] |
| animals | Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes [34359233] |
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