和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Copeptin (CPP)
C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin
- 編號UCEA365Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍24.69-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于9.16pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1361 ¥ 1944 ¥ 8748 ¥ 16524 ¥ 136080
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測和肽素(CPP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的和肽素(CPP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-97 | 81 |
| EDTA plasma(n=5) | 94-105 | 98 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的和肽素(CPP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中和肽素(CPP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-105% | 85-93% | 78-93% | 81-94% |
| EDTA plasma(n=5) | 79-103% | 97-105% | 91-98% | 82-105% |
| heparin plasma(n=5) | 79-90% | 88-102% | 99-105% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將和肽素(CPP)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA365Mu01 | 和肽素(CPP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCEA365Mu | 和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA365Mu | 和肽素(CPP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Med-Science. | Copeptin: May it be a novel biomarker for insulin theraphy in diabetes? An animal study. [Ejmanager: Source] |
| Psychiatry and Clinical Neurosciences | Serum copeptin in children exposed to maltreatment [Pubmed:27278269] |
| United States Patent Application | Methods for Diagnosis and Treatment of Concussion or Brain Injury [2016:0103140] |
| J Physiol | Role of enteroendocrine L‐cells in arginine vasopressin‐mediated inhibition of colonic anion secretion [Pubmed:27037495] |
| International Journal of Clinical and Experimental Medicine | Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … [files:ijcem0031358.pdf] |
| Front Physiol | Orthostatic Challenge Shifts the Hemostatic System of Patients Recovered from Stroke toward Hypercoagulability. [pubmed:28223937] |
| Kidney Blood Pressure Research | N-Terminal Prohormone of Brain Natriuretic Peptide but not C-Terminal Pre-Pro Vasopressin (Copeptin) Level is Associated with the Response to Antihypertensive … [pubmed:29190613] |
| Hindawi Publishing Corporation | Early Prediction of Preeclampsia [journals:297397] |
| Journal of Urology | Evaluation of Urinary Aquaporin 2 and Plasma Copeptin as Biomarkers of Effectiveness of Desmopressin Acetate for the Treatment of Monosymptomatic Nocturnal Enuresis. [pubmed:28457803] |
| American Journal of Physiology-Renal?Physiology | Experimental Heat Stress Nephropathy and Liver Injury are Improved by Allopurinol [Pubmed:29667911] |
| American Journal of Physiology-Regulatory Integrative and Comparative Physiology | Angiotensin AT Receptors Expressed in Vasopressin-Producing Cells of the Supraoptic Nucleus Contribute to the Osmotic Control of Vasopressin [Pubmed:29364700] |
| BMC?Nephrology | Rehydration with fructose worsens dehydration-induced renal damage [Pubmed:30005632] |
| Experimental and Therapeutic?Medicine | Copeptin level in the early prediction of cardiorenal syndrome in rats [Pubmed:30112047] |
| 中國病理生理雜志 | 和肽素水平對大鼠心腎綜合征的早期預(yù)測價值 [filename:ZBLS201802013] |
| Hypertension | Abstract P311: Static Urine Osmolality With Elevated First Trimester Urine Copeptin in Human Preeclampsia [] |
| Journal of Pediatric Urology | Assessment of serum levels of copeptin and corticotropin-releasing factor in children with monosymptomatic and nonmonosymptomatic nocturnal enuresis [] |
| experimental physiology | Cognitive performance is associated with cerebral oxygenation and peripheral oxygen saturation, but not plasma catecholamines, during graded normobaric hypoxia [] |
| international urology and nephrology | Dialysate copeptin and peritoneal transport in incident peritoneal dialysis patients [Pubmed: 31187425] |
| American Journal of Physiology-renal Physiology | The contribution of collecting duct NOS1 to the concentrating mechanisms in male and female mice [Pubmed: 31241990] |
| american journal of physiology-renal physiology | The increase of core temperature affected the progression of kidney injury by repeated heat stress exposure [Pubmed: 31390229] |
| Scientific Reports | Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion [Pubmed: 31863073] |
| BMC Nephrology | Copeptin is independently associated with vascular calcification in chronic kidney disease stage 5 [Pubmed: 32033584] |
| Archives of Biochemistry and Biophysics | Micro RNA 146a gene variant/TNF-α/IL-6/IL-1 β; A cross-link axis inbetween oxidative stress, endothelial dysfunction and neuro-inflammation in acute ischemic stroke … [Pubmed: 31738890] |
| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789] |
| Biomolecules . | Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study [Pubmed: 32549327] |
| Arch Med Res | Relation of Copeptin with Diabetic and Renal Function Markers Among Patients with Diabetes Mellitus Progressing Towards Diabetic Nephropathy [Pubmed: 32505416] |
| 河北醫(yī)科大學(xué)學(xué)報 | 重組人腦利鈉肽治療慢性肺心病急性加重期的臨床效果及對5-HT 濃度的影響 [] |
| Scientific reports | Validity of different copeptin assays in the differential diagnosis of the polyuria-polydipsia syndrome [33980941] |
| International Journal of Women?ˉs Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? [] |
| Dicle T?p Dergisi | Copeptin levels are more elevated and associated with cardiovascular events in the unity of chronic ischemic heart and chronic kidney diseases [] |
| High serum copeptin may be a marker of an increased carotid intima-media thickness in asymptomatic patients with type 1 diabetes. [] | |
| Nutrients | The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose [] |
| Acta Physiol (Oxf) | Dietary K+ acts as a genuine diuretic [Pubmed:34984847] |
| Anal Chim Acta | Spectrally multiplexed assay using gap enhanced nanoparticle for detection of a myocardial infarction biomarker panel [Pubmed:35190126] |
| J Cell Physiol | Arcuate NPY is involved in salt‐induced hypertension via modulation of paraventricular vasopressin and brain‐derived neurotrophic factor [Pubmed:35312067] |
| Children | Copeptin Concentrations in Plasma of Healthy Neonates in Relation to Water–Electrolyte Homeostasis in the Early Adaptation Period [Pubmed:35327814] |
