B-細胞淋巴瘤因子2(Bcl2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)
B-Cell CLL/Lymphoma 2
- 編號USEA778Po
- 物種Sus scrofa; Porcine (Pig，豬) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.054ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1588 ¥ 2268 ¥ 10206 ¥ 19278 ¥ 158760
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測B-細胞淋巴瘤因子2(Bcl2)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的B-細胞淋巴瘤因子2(Bcl2)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-101 | 96 |
| EDTA plasma(n=5) | 96-104 | 101 |
| heparin plasma(n=5) | 86-95 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的B-細胞淋巴瘤因子2(Bcl2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中B-細胞淋巴瘤因子2(Bcl2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-102% | 78-95% | 88-105% | 95-104% |
| EDTA plasma(n=5) | 86-101% | 84-101% | 85-93% | 94-102% |
| heparin plasma(n=5) | 78-101% | 94-101% | 83-90% | 78-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將B-細胞淋巴瘤因子2(Bcl2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的B-細胞淋巴瘤因子2(Bcl2)與連接于固相載體上的抗體結合，然后加入生物素化的B-細胞淋巴瘤因子2(Bcl2)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的B-細胞淋巴瘤因子2(Bcl2)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA778Po01 | B-細胞淋巴瘤因子2(Bcl2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA778Po01 | B-細胞淋巴瘤因子2(Bcl2)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA778Po | B-細胞淋巴瘤因子2(Bcl2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA778Po | B-細胞淋巴瘤因子2(Bcl2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233] |
| International Journal of Health Sciences | Neurotoxic effect of lead on rats: Relationship to Apoptosis [NCBI: PMC3883608] |
| Interactive cardiovascular and thoracic surgery | Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosin [Pubmed: 24327573] |
| J Physiol Biochem. | Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats [Pubmed:24752458] |
| Chem Biol Interact.? | Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activities [Pubmed:25289771] |
| Pediatric Cardiology | Serum Bcl-2 Values in Children with Pulmonary Hypertension [Pubmed:25381623] |
| Histochem Cell Biol. | Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis [Pubmed:25511416] |
| PLOS ONE | Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-γ, NF-κB and Nrf2/HO-1 Signaling Pathways [PubMed: 26196679] |
| Neurochem Res | Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436] |
| European Journal of Medicinal Plants | Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms [openview:626440] |
| IJBPAS | THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS [publication:298838934] |
| Mol Neurobiol. | Neuroprotective Effect of Nanodiamond in Alzheimer's Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling [Pubmed:26897372] |
| Iranian Journal of Basic Medical Sciences | The ameliorative potential of ethanolic extract of propolis on hematotoxicity and structural neuronal damage in hyperthermia-exposed rats [pubmed:27746870] |
| Scientific Reports | In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Disease [pubmed:27609215] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| Journal?of?Ethnopharmacology | Amelioration?of?cardiac?dysfunction?and?ventricular?remodeling?after?myocardial?infarction?bydanhong?injection?are?critically?contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms. [pubmed:27729285] |
| European Journal of Pharmacology | Mangiferin protects against? intestinal ischemia/reperfusion-induced? liver injury:?? Involvement of PPAR-? γ, GSK-3β and Wnt/β-catenin pathway???????????????????????????????????????????????? [pubmed:28506911] |
| Journal of Applied Pharmaceutical Science | Comparative Effects of Stevia rebaudiana and Aspartame on hepato-renal function of diabetic rats: Biochemical and Histological Approaches [59b5141c458515a5b49377cf] |
| Cellular Physiology and Biochemistry | A Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in Rats [pubmed:28942443] |
| Biomedicine & Pharmacotherapy | Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in rats [pubmed:28554128] |
| Thromb Haemost | Distinctive expression signatures of serum microRNAs in ischaemic stroke and transient ischaemic attack patients [pubmed:28251236] |
| International Journal of Biological Macromolecules | A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats [pubmed:28709897] |
| Molecular Neurobiology | Neuroprotective Effect of Nanodiamond in Alzheimer’s Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling [pubmed:26897372] |
| Toxicology and Environmental Health Sciences | Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin [10.1007/s13530-017-0325-1] |
| Biomedicine & Pharmacotherapy | Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heart [pubmed:28686977] |
| Scientific Research | Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress Disorder [paperID:82598] |
| Biomaterials | Structural optimization and additional targets identification of antisense oligonucleotide G3139 encapsulated in a neutral cytidinyl-lipid combined with a cationic lipid?… [Pubmed: 30660994] |
| PeerJ. | Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway [Pubmed: 30723634] |
| 博士論文 | INSTITUTO DE CIENCIAS BáSICAS Y PRECLíNICAS “VICTORIA DE GIRóN” DEPARTAMENTO DE HISTOLOGíA [] |
| pharmacological research | Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors [Pubmed: 31048034] |
| Int Immunopharmacol | Metallothionein-1 is associated with osteoarthritis disease activity and suppresses proinflammatory cytokines production in synovial cells [Pubmed: 31465913] |
| Experimental Cell Research | LncRNA SNHG3 promotes clear cell renal cell carcinoma proliferation and migration by upregulating TOP2A [Pubmed: 31505165] |
| Toxicology | LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating … [Pubmed: 32197949] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
| Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453] |
| Drug Chem Toxicol | Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascade [Pubmed: 32869669] |
| Iranian Journal of phamaceutical research | Potential anti-tumor activity of kefir-induced juglone and resveratrol fractions against Ehrlich ascites carcinoma-bearing BALB/c mice [] |
| ACS APPLIED BIO MATERIALS | Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy [] |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats [] |
| Current Nutrition & Food Science | The Effect of Sodium Metabisulphite on Apoptosis in the Experimental Model of Parkinson's Disease [] |
| Life Sci | Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-|?, NF-|êB, and STAT3 crosstalk [33310046] |
| J Biochem Mol Toxicol | Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ?- [33491863] |
| Drug Des Devel Ther | Magnesium Isoglycyrrhizinate Alleviates Arsenic Trioxide-Induced Cardiotoxicity: Contribution of Nrf2 and TLR4/NF-|êB Signaling Pathway [33603344] |
| Crystals | Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent) [] |
| BIOTECHNOLOGIES | Expression of Apoptotic Markers Bcl-2 and Bax in the Vascular Wall [] |
| Pharmacol Res Perspect | [8]‐Gingerol exerts anti‐myocardial ischemic effects in rats via modulation of the MAPK signaling pathway and L‐type Ca2+ channels [34390539] |
| Ecotoxicol Environ Saf | Lycopene attenuates zearalenone-induced oxidative damage of piglet sertoli cells through the nuclear factor erythroid-2 related factor signaling pathway [34482067] |
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats [34426252] |
| Environ Sci Pollut Res Int | Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity [34729716] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498] |
| Life Sci | Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and?… [Pubmed:35640776] |
| Life Sci | Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosis [Pubmed:35636581] |
| Antioxidants (Basel) | Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino?… [Pubmed:35624893] |
| Experimental and Therapeutic Medicine | Nephroprotective effect of magnesium isoglycyrrhizinate against arsenic trioxide?induced acute kidney damage in mice [Pubmed:35317438] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
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