抗繆勒管激素(AMH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Anti-Mullerian Hormone (AMH)
MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance
- 編號UCEA228Ov
- 物種Ovis aries; Ovine (Sheep，綿羊) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長2h
- 檢測范圍123.5-10,000pg/mL
- 靈敏度最小可檢測劑量小于等于49.3pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1701 ¥ 2430 ¥ 10935 ¥ 20655 ¥ 170100
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測抗繆勒管激素(AMH)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的抗繆勒管激素(AMH)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-94 | 85 |
| EDTA plasma(n=5) | 81-95 | 87 |
| heparin plasma(n=5) | 78-96 | 92 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的抗繆勒管激素(AMH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中抗繆勒管激素(AMH)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-99% | 85-98% | 80-101% | 78-99% |
| EDTA plasma(n=5) | 92-101% | 90-104% | 91-101% | 88-95% |
| heparin plasma(n=5) | 95-105% | 80-95% | 92-101% | 79-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將抗繆勒管激素(AMH)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Ovis aries; Ovine (Sheep，綿羊) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCEA228Ov | 抗繆勒管激素(AMH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Zhejiang University SCIENCE B | Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. [PubMed: 23125082] |
| In Vivo.(Athens,Greece) | Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF [Pubmed: 24292596] |
| Gynecological Endocrinology | The effects of isotretinoin on the ovarian reserve of females with acne [Informahealthcare: Source] |
| Fertility and Sterility | Increased expression of anti mullerian hormone and its receptor in endometriosis [Ingentaconnect: Source] |
| Reproductive BioMedicine Online | Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure [Rbmojournal: Source] |
| Reproductive Biology and Endocrinology | The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. [Pubmed: 24341292] |
| Biomed Res Int. | Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats [Pubmed:Pmc4053297] |
| Fertil Steril. | Increased expression of antimüllerian hormone and its receptor in endometriosis [Pubmed:24613539] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Maternal serum anti-Müllerian hormone at 11-13 weeks' gestation in the prediction of preeclampsia [Pubmed:24953352] |
| J Gynecol Oncol. | Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model [Pubmed:25142624] |
| Reproductive BioMedicine Online | The effect of childhood cancer therapy on ovarian reserve and pubertal development [Pubmed:25498597] |
| Reproductive Sciences | Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome [Pubmed:25667201] |
| Yonsei Medical Journal | Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines [PubMed: 26632380] |
| oncology research | Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats [PubMed: 26629940] |
| Reprod Biomed Online | Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats [PubMed: 26206282] |
| PLoS One | Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis [PubMed: 26465611] |
| Toxicol Lett | Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood [PubMed: 26657179] |
| Journal of Assisted Reproduction and Genetics | Can?Coenzyme?Q10?supplementation?protect?the?ovarian?reserve?against?oxidative?damage? [Pubmed:27255570] |
| Anticancer Res. | Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer [Pubmed:26976997] |
| Chemosphere. | Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. [pubmed:27567155] |
| Theriogenology | Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. [pubmed:28043339] |
| environmental pollution | Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061] |
| Journal of Assisted Reproduction and Genetics | Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. [pubmed:28074436] |
| Journal?Pakistan?Medical?Association | Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice [pubmed:27654725] |
| Advance?Medical?Associates | Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? [pubmed:27162065] |
| Yonsei?Med?J Volume | Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. [pubmed:26632380] |
| Journal?of?Endocrinological?Investigation | The use of?serum?anti-Mullerian?hormone?(AMH)?levels?and?antral?follicle?count?(AFC) to?predictthe?number?of?oocytes?collected?and?availability?of?embryos?for cryopreservation in IVF. [pubmed:27465668] |
| Journal of the Pakistan Medical Association | Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice [pubmed:28955058] |
| International Journal of Reproduction, Contraception, Obstetrics and Gynecology | Can Co-enzyme Q10 improve the chances of conception after the age of 35? [10.18203/2320-1770.ijrcog20172902] |
| Animal Production Science | Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? [10.1071/ANv57n12Ab065] |
| Reproduction. | Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring [pubmed:28687593] |
| Theriogenology | Relationship among circulating anti-Müllerian hormone, insulin like growth factor 1, cadmium and superovulatory response in dairy cows. [pubmed:28708536] |
| Journal of ovarian research | Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo. [pubmed:28814333] |
| Annals of Rheumatic Diseases? | FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study [Suppl_2:728.1.abstract] |
| Free?Radical?Biology?&?Medicine | Peroxiredoxin 2 deficiency accelerates age-related ovarian failure through the reactive oxygen species-mediated JNK pathway in mice [Pubmed:29778464] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
| Biology of Reproduction | Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss [Pubmed:29351580] |
| Endocrine?Connections | Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery [Pubmed:30012648] |
| Reproductive BioMedicine?Online | Dienogest suppresses the activation of primordial follicles and preserves the primordial follicle stockpile for fertility in mice [Pubmed:29609766] |
| Journal of?Ovarian?Research | Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian?… [Pubmed:29859107] |
| Animal?Reproduction?Science | Anti-Müllerian hormone and Oestradiol as markers of future reproductive success in juvenile gilts [Pubmed:29859701] |
| Stem?Cell?Research?&?Therapy | Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the?… [Pubmed: 30606243] |
| Biochemical?and?Biophysical?Research?Communications | Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells [Pubmed: 30722991] |
| Lupus | Levels of anti-Müllerian hormone in premenopausal women with the antiphospholipid syndrome and its association with the risk of clinical complications [Pubmed: 30717622] |
| In Vitro Cellular & Developmental Biology - Animal | Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice [Pubmed: 30820812] |
| Biomedicine & Pharmacotherapy | Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve [] |
| Reproductive Biology and Endocrinology | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in?… [Pubmed: 31311554] |
| Oxidative Medicine and Cellular Longevity | Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure [] |
| Acta Obstet Gynecol Scand | The impact of uterine artery embolization on ovarian reserve: a systematic review and meta‐analysis [Pubmed: 31370100] |
| Reproductive Sciences | Uterine Cells Improved Ovarian Function in a Murine Model of Ovarian Insufficiency [Pubmed: 31530098] |
| Animals | Serum Concentrations of AMH and E2 and Ovarian and Uterine Traits in Gilts [Pubmed: 31619004] |
| Journal Of Pakistan Medical Association | The effect of acupuncture on anti-mullerian hormone and assisted reproduction outcome in Polycystic Ovary Syndrome patients undergoing in vitro fertilization?… [Pubmed: 31603867] |
| In Vitro Cellular & Developmental Biology | Basal characterization and in vitro differentiation of putative stem cells derived from the adult mouse ovary [Pubmed: 31900800] |
| J Assist Reprod Genet | The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage [Pubmed: 32020412] |
| Biology (Basel). | Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker? [Pubmed: 32290279] |
| Eur J Breast Health | The Effect of Systemic Chemotherapy on Ovarian Function: A Prospective Clinical Trial [Pubmed: 32656517] |
| Heliyon | Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting?… [Pubmed: 33005806] |
| CYTOMETRY PART A | After cyclophosphamide exposure, granulosa cells recover their anti?\M¨1llerian hormone?\producing ability but not their numbers [33342073] |
| BIOLOGY OF REPRODUCTION | Igf3 is essential for ovary differentiation in zebrafish [33276384] |
| JOURNAL OF ETHNOPHARMACOLOGY | Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655] |
| Stem Cells Int | Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in?… [34733331] |
| Sci Total Environ | Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression [Pubmed:35276145] |
| Hum Reprod Update | Anti-Müllerian hormone as a marker of ovarian reserve and premature ovarian insufficiency in children and women with cancer: a systematic review [Pubmed:35199161] |
| Horm Metab Res | Anti-Mullerian Hormone Levels Increase After Bariatric Surgery in Obese Female Patients With and Without Polycystic Ovary Syndrome [Pubmed:35276745] |
| American Journal of Translational Research | The effects of vasopressin injection technique on ovarian reserve in laparoscopic cystectomy of bilateral ovarian endometrioma: a retrospective cohort study [Pubmed:35559399] |
| Stem Cell Research & Therapy | G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and?… [] |
| Research Square | Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency [] |
| Fundamental Research | Long-term amelioration of an early-onset familial atrial fibrillation model with AAV-mediated in vivo gene therapy [] |
留言咨詢
